Overview
- External ontology
- mediadive.medium:J947 (source link unavailable) — CALDICELLULOSIRUPTOR MEDIUM WITH 2.5% NaCl
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=947
Composition
flowchart LR
CultureMech_003295["caldicellulosiruptor_medium_with_2_5_nacl"]
ing0_NH4Cl["NH4Cl"]
CultureMech_003295 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_NaCl["NaCl"]
CultureMech_003295 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003295 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_KH2PO4["KH2PO4"]
CultureMech_003295 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_003295 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Trypticase_peptone["Trypticase peptone"]
CultureMech_003295 --> ing5_Trypticase_peptone
ing6_Yeast_extract["Yeast extract"]
CultureMech_003295 --> ing6_Yeast_extract
ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003295 --> ing7_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
ing8_Cellobiose["Cellobiose"]
CultureMech_003295 --> ing8_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing8_Cellobiose -.-> CHEBI_17057
ing9_Glucose["Glucose"]
CultureMech_003295 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003295 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Resazurin["Resazurin"]
CultureMech_003295 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_HCl["HCl"]
CultureMech_003295 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003295 --> ing13_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing13_FeCl2_x_4_H2O -.-> CHEBI_86249
ing14_ZnCl2["ZnCl2"]
CultureMech_003295 --> ing14_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing14_ZnCl2 -.-> CHEBI_49976
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003295 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_H3BO3["H3BO3"]
CultureMech_003295 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003295 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003295 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003295 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003295 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.899101 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
24.975 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.3996 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.749251 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.4985 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 | Trypticase peptone | 1.998 G_PER_L | ||
| 7 | Yeast extract | 0.999001 G_PER_L | ||
| 8 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.0024975 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 9 |
Cellobiose
C12H22O11 342.297 g/mol
|
0.999001 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
0.999001 G_PER_L |
CHEBI:17234
glucose
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.749251 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0004995 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 14 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 15 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.5.
- Prepare medium anaerobically under 100% N2. Add cellobiose after autoclaving from an anoxic stock solution prepared under N2 gas atmosphere and sterilized by filtration.
- Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.094573Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J947 |
| 2026-03-13T06:19:19.284875Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003295 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.217958+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CALDICELLULOSIRUPTOR MEDIUM WITH 2.5% NaCl" to "caldicellulosiruptor_medium_with_2_5_nacl" for consistent matching |
| 2026-06-05T07:40:52.098674+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.149212+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |