gauzes_synthetic_medium_no_1_ph_5_3 (CultureMech:003296)

Overview

External ontology

mediadive.medium:J949 (source link unavailable) — GAUZE'S SYNTHETIC MEDIUM NO. 1 (pH 5.3)

Applications

Microbial cultivation

pH

5.3

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=949

Composition

flowchart LR
    CultureMech_003296["gauzes_synthetic_medium_no_1_ph_5_3"]
    ing0_Starch["Starch"]
    CultureMech_003296 --> ing0_Starch
    CHEBI_28017["CHEBI:28017"]
    ing0_Starch -.-> CHEBI_28017
    ing1_KNO3["KNO3"]
    CultureMech_003296 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_NaCl["NaCl"]
    CultureMech_003296 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003296 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_K2HPO4["K2HPO4"]
    CultureMech_003296 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003296 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_Agar["Agar"]
    CultureMech_003296 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

#	Ingredient	Concentration	Ontology	Notes
1	Starch	20 G_PER_L	CHEBI:28017 starch	Copied from referenced JCM Medium 81
2	KNO3 K.NO3 101.102 g/mol	1 G_PER_L	CHEBI:63043 potassium nitrate	Copied from referenced JCM Medium 81
3	NaCl Cl.Na 58.443 g/mol	0.5 G_PER_L	CHEBI:26710 sodium chloride	Copied from referenced JCM Medium 81
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.5 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Copied from referenced JCM Medium 81
5	K2HPO4 HO4P.2K 174.174 g/mol	0.5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Copied from referenced JCM Medium 81
6	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.01 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Copied from referenced JCM Medium 81
7	Agar	15 G_PER_L	CHEBI:2509 agar	Copied from referenced JCM Medium 81

Preparation

Prepare Gauze's synthetic medium No. 1 (see Medium No. 81) and adjust pH to 5.3.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:18.103957Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J949
2026-02-03T11:26:56.734432	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:19.289640Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:003296
2026-03-17T19:45:33.223591+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "GAUZE'S SYNTHETIC MEDIUM NO. 1 (pH 5.3)" to "gauzes_synthetic_medium_no_1_ph_5_3" for consistent matching
2026-04-04T21:48:41.228043	resolve_option_c_references	RESOLVED_REFERENCE	Resolved reference by copying from gauzes_synthetic_medium_no_1 (CultureMech:003164)
2026-04-04T21:55:01.098716	resolve_option_c_references	RESOLVED_REFERENCE	Resolved reference by copying from gauzes_synthetic_medium_no_1 (CultureMech:003164)
2026-05-16T08:57:33.759837+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(2 keys) -> list(1 entries)
2026-06-05T07:41:05.953073+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.903792+00:00	primary-term-regrounding-v1.0	Re-grounded wrong primary term id (OAK-verified)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:58.957674+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.