Overview
- External ontology
- mediadive.medium:J950 (source link unavailable) — NH-JN4 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=950
Composition
flowchart LR
CultureMech_003298["nh_jn4_medium"]
ing0_Na2SO4["Na2SO4"]
CultureMech_003298 --> ing0_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing0_Na2SO4 -.-> CHEBI_32149
ing1_NaCl["NaCl"]
CultureMech_003298 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_KCl["KCl"]
CultureMech_003298 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003298 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003298 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_K2HPO4["K2HPO4"]
CultureMech_003298 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_KH2PO4["KH2PO4"]
CultureMech_003298 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NH4Cl["NH4Cl"]
CultureMech_003298 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_003298 --> ing8_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing8_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing9_Yeast_extract["Yeast extract"]
CultureMech_003298 --> ing9_Yeast_extract
ing10_Tryptone["Tryptone"]
CultureMech_003298 --> ing10_Tryptone
ing11_Sodium_acetate["Sodium acetate"]
CultureMech_003298 --> ing11_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing11_Sodium_acetate -.-> CHEBI_32954
ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003298 --> ing12_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing13_Resazurin["Resazurin"]
CultureMech_003298 --> ing13_Resazurin
CHEBI_8806["CHEBI:8806"]
ing13_Resazurin -.-> CHEBI_8806
ing14_NaHCO3["NaHCO3"]
CultureMech_003298 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
ing15_HCl["HCl"]
CultureMech_003298 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003298 --> ing16_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
ing17_ZnCl2["ZnCl2"]
CultureMech_003298 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003298 --> ing18_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
ing19_H3BO3["H3BO3"]
CultureMech_003298 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003298 --> ing20_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003298 --> ing21_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003298 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003298 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.999001 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
29.97 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.699301 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.99501 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.999001 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3996 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3996 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.499501 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.001998 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 10 | Yeast extract | 2.997 G_PER_L | ||
| 11 | Tryptone | 3.996 G_PER_L | ||
| 12 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
6.79321 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 13 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.599401 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 14 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000999001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
40 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 16 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 17 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 19 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 22 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components thoroughly and adjust pH to 6.8. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 960 ml) from anaerobic stock (filter-sterilized)
- Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.105953Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J950 |
| 2026-01-28T19:48:31.063040Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:19.300178Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003298 |
| 2026-03-17T06:30:31.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.234828+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NH-JN4 MEDIUM" to "nh_jn4_medium" for consistent matching |
| 2026-06-05T07:41:39.642763+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.541551+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |