Overview
- External ontology
- mediadive.medium:J967 (source link unavailable) — KD-1 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=967
Composition
flowchart LR
CultureMech_003315["kd_1_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_003315 --> ing0_Bacto_peptone
ing1_Urea["Urea"]
CultureMech_003315 --> ing1_Urea
CHEBI_16199["CHEBI:16199"]
ing1_Urea -.-> CHEBI_16199
ing2_K2HPO4["K2HPO4"]
CultureMech_003315 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KH2PO4["KH2PO4"]
CultureMech_003315 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NaCl["NaCl"]
CultureMech_003315 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003315 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Resazurin["Resazurin"]
CultureMech_003315 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Biotin["Biotin"]
CultureMech_003315 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Folic_acid["Folic acid"]
CultureMech_003315 --> ing8_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing8_Folic_acid -.-> CHEBI_27470
ing9_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003315 --> ing9_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing9_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing10_Thiamine_HCl["Thiamine HCl"]
CultureMech_003315 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_Riboflavin["Riboflavin"]
CultureMech_003315 --> ing11_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing11_Riboflavin -.-> CHEBI_17015
ing12_Nicotinic_acid["Nicotinic acid"]
CultureMech_003315 --> ing12_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing12_Nicotinic_acid -.-> CHEBI_15940
ing13_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003315 --> ing13_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing13_Calcium_pantothenate -.-> CHEBI_31345
ing14_Vitamin_B12["Vitamin B12"]
CultureMech_003315 --> ing14_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing14_Vitamin_B12 -.-> CHEBI_176843
ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003315 --> ing15_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
ing16_Lipoic_acid["Lipoic acid"]
CultureMech_003315 --> ing16_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing16_Lipoic_acid -.-> CHEBI_16494
ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003315 --> ing17_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
ing18_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003315 --> ing18_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing18_MnSO4_x_n_H2O -.-> CHEBI_86360
ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003315 --> ing19_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003315 --> ing20_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
ing21_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003315 --> ing21_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing21_CaCl2_x_2_H2O -.-> CHEBI_86158
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003315 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003315 --> ing23_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
ing24_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_003315 --> ing24_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing24_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing25_H3BO3["H3BO3"]
CultureMech_003315 --> ing25_H3BO3
CHEBI_33118["CHEBI:33118"]
ing25_H3BO3 -.-> CHEBI_33118
ing26_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003315 --> ing26_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing26_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing27_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003315 --> ing27_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing27_NiCl2_x_6_H2O -.-> CHEBI_34887
ing28_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003315 --> ing28_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing28_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 9.80392 G_PER_L | ||
| 2 |
Urea
CH4N2O 60.056 g/mol
|
1.96078 G_PER_L |
CHEBI:16199
urea
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.45098 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.94118 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
3.94118 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 2.94118, 1.0] |
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.196078 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.196078, 3.0] |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000980392 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 9 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 10 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 11 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 12 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 13 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 14 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 15 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 16 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 17 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 18 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 19 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 20 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 21 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 22 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 24 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 25 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 26 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 27 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 28 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 29 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Mix components thoroughly and adjust pH to 8.0. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
- Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH, then add the remaining minerals.
- Adjust final pH of the medium to 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.159971Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J967 |
| 2026-03-13T03:09:37.491418Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:19.438392Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003315 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.396810+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KD-1 MEDIUM" to "kd_1_medium" for consistent matching |
| 2026-06-05T07:41:10.350270+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.353657+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |