Overview
- External ontology
- mediadive.medium:J96 (source link unavailable) — AE BROTH
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=96
Composition
flowchart LR
CultureMech_003318["ae_broth"]
ing0_Glucose["Glucose"]
CultureMech_003318 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Yeast_extract["Yeast extract"]
CultureMech_003318 --> ing1_Yeast_extract
ing2_Peptone["Peptone"]
CultureMech_003318 --> ing2_Peptone
ing3_Acetic_acid["Acetic acid"]
CultureMech_003318 --> ing3_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing3_Acetic_acid -.-> CHEBI_15366
ing4_Ethanol["Ethanol"]
CultureMech_003318 --> ing4_Ethanol
CHEBI_16236["CHEBI:16236"]
ing4_Ethanol -.-> CHEBI_16236
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
15.2284 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Yeast extract | 2.03046 G_PER_L | ||
| 3 | Peptone | 3.04569 G_PER_L | ||
| 4 |
Acetic acid
C2H4O2 60.052 g/mol
|
65 G_PER_L |
CHEBI:15366
acetic acid
|
|
| 5 |
Ethanol
C2H6O 46.069 g/mol
|
20 G_PER_L |
CHEBI:16236
ethanol
|
Preparation
- Mix ingredients, except acetic acid and ethanol, and autoclave. Aseptically add acetic acid and ethanol to the medium. Unadjusted pH will be about 2.9.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.723860Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J96 |
| 2026-03-13T06:19:19.456984Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003318 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.419355+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AE BROTH" to "ae_broth" for consistent matching |
| 2026-06-05T07:40:47.500912+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |