1_2_sme_medium_for_ya

CultureMech:003320 bacterial COMPLEX LIQUID
Original name: 1/2 SME MEDIUM FOR YA

Overview

External ontology
mediadive.medium:J971 (source link unavailable) — 1/2 SME MEDIUM FOR YA
Applications
  • Microbial cultivation
pH
1.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=971

Composition

flowchart LR
    CultureMech_003320["1_2_sme_medium_for_ya"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003320 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_003320 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Sodium_acetate_trihydrate["Sodium acetate trihydrate"]
    CultureMech_003320 --> ing2_Sodium_acetate_trihydrate
    CHEBI_32138["CHEBI:32138"]
    ing2_Sodium_acetate_trihydrate -.-> CHEBI_32138
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_003320 --> ing3_Yeast_extract
    ing4_Modified_Wolfe_s_minerals["Modified Wolfe's minerals"]
    CultureMech_003320 --> ing4_Modified_Wolfe_s_minerals
    ing5_Resazurin["Resazurin"]
    CultureMech_003320 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_NaHCO3["NaHCO3"]
    CultureMech_003320 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003320 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003320 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003320 --> ing9_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing10_NaCl["NaCl"]
    CultureMech_003320 --> ing10_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing10_NaCl -.-> CHEBI_26710
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003320 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003320 --> ing12_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003320 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003320 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003320 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_003320 --> ing16_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing17_H3BO3["H3BO3"]
    CultureMech_003320 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003320 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_003320 --> ing19__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing19__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing20_Na2SeO4["Na2SeO4"]
    CultureMech_003320 --> ing20_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing20_Na2SeO4 -.-> CHEBI_77775
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003320 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003320 --> ing22_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing22_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing23_KCl["KCl"]
    CultureMech_003320 --> ing23_KCl
    CHEBI_32588["CHEBI:32588"]
    ing23_KCl -.-> CHEBI_32588
    ing24_NaBr["NaBr"]
    CultureMech_003320 --> ing24_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing24_NaBr -.-> CHEBI_63004
    ing25_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_003320 --> ing25_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing25_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing26_KI["KI"]
    CultureMech_003320 --> ing26_KI
    CHEBI_8346["CHEBI:8346"]
    ing26_KI -.-> CHEBI_8346
    ing27_Biotin["Biotin"]
    CultureMech_003320 --> ing27_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_Biotin -.-> CHEBI_15956
    ing28_Folic_acid["Folic acid"]
    CultureMech_003320 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470
    ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003320 --> ing29_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
    CultureMech_003320 --> more["...7 more"]

Ingredients (37)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.451671 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.271003 G_PER_L CHEBI:31206
ammonium chloride
3 Sodium acetate trihydrate
C2H3O2.3H2O.Na 136.079 g/mol
0.97561 G_PER_L CHEBI:32138
sodium acetate trihydrate
4 Yeast extract 0.180668 G_PER_L
5 Modified Wolfe's minerals 1 G_PER_L
6 Resazurin
C12H7NO4 229.191 g/mol
0.000903342 G_PER_L CHEBI:8806
Resazurin
7 NaHCO3
CHO3.Na 84.006 g/mol
2.71003 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
16.71003 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 2.71003, 14.0]
10 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.451671 G_PER_L CHEBI:86360
manganese(II) sulfate
11 NaCl
Cl.Na 58.443 g/mol
56.303342 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.903342, 55.4]
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0903342 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.162602 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
14 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.5903342 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0903342, 1.5]
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.162602 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.00903342 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.0162602 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.06903342 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.00903342, 0.06]
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.00903342 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
0.252936 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
21 Na2SeO4
2Na.O4Se 188.936 g/mol
0.00903342 G_PER_L CHEBI:77775
sodium selenate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.00903342 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
11 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
24 KCl
Cl.K 74.551 g/mol
1.3 G_PER_L CHEBI:32588
potassium chloride
25 NaBr
Br.Na 102.894 g/mol
0.2 G_PER_L CHEBI:63004
sodium bromide
26 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.03 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
27 KI
I.K 166.002 g/mol
0.0001 G_PER_L CHEBI:8346
potassium iodide
28 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
30 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
31 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
32 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
33 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
34 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
35 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
36 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
37 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly, boil for a few seconds and cool down under a stream of a N2-CO2(4:1, v/v) gas mixture. After cooling, add the following components and adjust pH to 7.0:
  2. Dispense the medium into culture vessels anaerobically under the same gas mixture, seal with butyl rubber stoppers and autoclave.
  3. <I>Modified Wolfe's minerals (10 x): </I>
  4. Adjust pH to 1.0 with 1 N H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.172962Z mediadive-import Imported from MediaDive Source: JCM, ID: J971
2026-03-13T03:08:55.810255Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:19.475578Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003320
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.461312+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/2 SME MEDIUM FOR YA" to "1_2_sme_medium_for_ya" for consistent matching
2026-06-05T07:40:15.546272+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.047816+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:42.503485+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.