Overview
- External ontology
- mediadive.medium:J972 (source link unavailable) — ARTIFICIAL SEAWATER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=972
Composition
flowchart LR
CultureMech_003321["artificial_seawater_medium"]
ing0_NaCl["NaCl"]
CultureMech_003321 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_003321 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003321 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Yeast_extract["Yeast extract"]
CultureMech_003321 --> ing3_Yeast_extract
ing4_Peptone["Peptone"]
CultureMech_003321 --> ing4_Peptone
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
24 G_PER_L |
CHEBI:26710
sodium chloride
|
Copied from referenced JCM Medium 701 |
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.7 G_PER_L |
CHEBI:32588
potassium chloride
|
Copied from referenced JCM Medium 701 |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
7 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Copied from referenced JCM Medium 701 |
| 4 | Yeast extract | 0.1 G_PER_L | Copied from referenced JCM Medium 701 | |
| 5 | Peptone | 0.5 G_PER_L | Copied from referenced JCM Medium 701 |
Preparation
- Use Medium No. 701 with 3.0 g/L (final) yeast extract and 2.5 g/L (final) peptone.
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.178684Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J972 |
| 2026-02-03T11:26:56.833800 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:19.482754Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003321 |
| 2026-03-17T19:45:33.478623+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ARTIFICIAL SEAWATER MEDIUM" to "artificial_seawater_medium" for consistent matching |
| 2026-04-04T18:49:19.936468 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from low_strength_artificial_seawater_medium (CultureMech:003047) |
| 2026-05-16T08:57:23.765188+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:49.763642+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |