salinivibrio_sharmensis_medium

CultureMech:003324 bacterial COMPLEX LIQUID
Original name: SALINIVIBRIO SHARMENSIS MEDIUM

Overview

External ontology
mediadive.medium:J975 (source link unavailable) — SALINIVIBRIO SHARMENSIS MEDIUM
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=975

Composition

flowchart LR
    CultureMech_003324["salinivibrio_sharmensis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003324 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Trisodium_citrate["Trisodium citrate"]
    CultureMech_003324 --> ing1_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing1_Trisodium_citrate -.-> CHEBI_53258
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003324 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_003324 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003324 --> ing4_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003324 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_003324 --> ing6_Yeast_extract
    ing7_Na2CO3["Na2CO3"]
    CultureMech_003324 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
2 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
2 G_PER_L CHEBI:32588
potassium chloride
5 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.00036 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.05 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 Yeast extract 10 G_PER_L
8 Na2CO3
CO3.2Na 105.988 g/mol
3 G_PER_L CHEBI:29377
sodium carbonate

Preparation

  1. Add components to distilled water and bring volume to 970 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add the following solution (separately autoclaved):
  2. Check the final pH to be around 9.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.184941Z mediadive-import Imported from MediaDive Source: JCM, ID: J975
2026-03-13T06:19:19.498590Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003324
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.507364+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SALINIVIBRIO SHARMENSIS MEDIUM" to "salinivibrio_sharmensis_medium" for consistent matching
2026-06-05T07:40:17.943527+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.189577+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:52:40.011908+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.