mesanospirillum_x_18_medium

CultureMech:003333 bacterial COMPLEX LIQUID
Original name: MESANOSPIRILLUM X-18 MEDIUM

Overview

External ontology
mediadive.medium:J984 (source link unavailable) — MESANOSPIRILLUM X-18 MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=984

Composition

flowchart LR
    CultureMech_003333["mesanospirillum_x_18_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003333 --> ing0_Yeast_extract
    ing1_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003333 --> ing1_Trypticase_peptone
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003333 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003333 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003333 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_K2HPO4["K2HPO4"]
    CultureMech_003333 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_NaHCO3["NaHCO3"]
    CultureMech_003333 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Resazurin["Resazurin"]
    CultureMech_003333 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003333 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003333 --> ing9_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing10_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_003333 --> ing10_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing10_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing11_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003333 --> ing11_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing11_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003333 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003333 --> ing13_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing13_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing14_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003333 --> ing14_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing14_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003333 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003333 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003333 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlCl3["AlCl3"]
    CultureMech_003333 --> ing18_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing18_AlCl3 -.-> CHEBI_30114
    ing19_H3BO3["H3BO3"]
    CultureMech_003333 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003333 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003333 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003333 --> ing22_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003333 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Riboflavin["Riboflavin"]
    CultureMech_003333 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003333 --> ing25_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing25_Thiamine_HCl -.-> CHEBI_49105
    ing26_Biotin["Biotin"]
    CultureMech_003333 --> ing26_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing26_Biotin -.-> CHEBI_15956
    ing27_Folic_acid["Folic acid"]
    CultureMech_003333 --> ing27_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing27_Folic_acid -.-> CHEBI_27470
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_003333 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494
    ing29_Vitamin_B12["Vitamin B12"]
    CultureMech_003333 --> ing29_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing29_Vitamin_B12 -.-> CHEBI_176843

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 Yeast extract 3.84615 G_PER_L
2 Trypticase peptone 1.92308 G_PER_L
3 NH4Cl
Cl.H4N 53.492 g/mol
0.961538 G_PER_L CHEBI:31206
ammonium chloride
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.961538 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.384615 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 K2HPO4
HO4P.2K 174.174 g/mol
0.384615 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 NaHCO3
CHO3.Na 84.006 g/mol
0.480769 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Resazurin
C12H7NO4 229.191 g/mol
0.000480769 G_PER_L CHEBI:8806
Resazurin
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
10 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
11 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.2 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
12 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.2 G_PER_L CHEBI:131361
disodium selenite pentahydrate
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
15 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.1 G_PER_L CHEBI:86360
manganese(II) sulfate
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlCl3
AlCl3 133.341 g/mol
0.04 G_PER_L CHEBI:30114
aluminium trichloride
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 Nicotinic acid
C6H5NO2 123.111 g/mol
0.01 G_PER_L CHEBI:15940
nicotinic acid
23 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
26 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.01 G_PER_L CHEBI:49105
thiamine hydrochloride
27 Biotin
C10H16N2O3S 244.316 g/mol
0.005 G_PER_L CHEBI:15956
biotin
28 Folic acid
C19H19N7O6 441.404 g/mol
0.005 G_PER_L CHEBI:27470
folic acid
29 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
30 Vitamin B12 0.005 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Mix components thoroughly. Distribute medium into culture vessels under a stream of H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (filter-sterilized):
  2. Prior to inoculation, reduce the medium by the addition of the following solution (per liter, autoclaved and stored under a N2 atmosphere):
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
  4. Comment: Incubate with a 10 to 20% inoculum.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.216016Z mediadive-import Imported from MediaDive Source: JCM, ID: J984
2026-01-28T19:48:31.553235Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:19.574308Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003333
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.596984+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MESANOSPIRILLUM X-18 MEDIUM" to "mesanospirillum_x_18_medium" for consistent matching
2026-06-05T07:41:30.494240+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.581923+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.