Overview
- External ontology
- mediadive.medium:J986 (source link unavailable) — METHANOCOCCOIDES MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=986
Composition
flowchart LR
CultureMech_003335["methanococcoides_medium"]
ing0_Trimethylamine_hydrochloride["Trimethylamine hydrochloride"]
CultureMech_003335 --> ing0_Trimethylamine_hydrochloride
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trimethylamine hydrochloride | 3 G_PER_L |
Preparation
- Use Medium No. 265 supplemented with 3.0 g/L trimethylamine x HCl and adjust pH to 7.0 - 7.2. Prepare the medium and cultivate under a N2-CO2 (80:20, v/v) gas mixture.
Solutions
METHANOGENIUM MEDIUM (JCM 265)
Restore the source's base-medium reference instead of 37 flattened copied concentrations, which conflate stock stages and include duplicate sums. The base constituents are not excluded from the medium: they remain in the linked base recipe. Its inherited concentrations remain unverified. JCM986 specifies no numeric base working dose here; none is inferred from TOGO M1039.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.225425Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J986 |
| 2026-02-03T11:26:56.893219 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:19.594760Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003335 |
| 2026-03-17T19:45:33.621383+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOCOCCOIDES MEDIUM" to "methanococcoides_medium" for consistent matching |
| 2026-04-04T18:49:19.308870 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from methanogenium_medium (CultureMech:000295) |
| 2026-05-16T08:57:05.029535+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:17.965190+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.194644+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T10:47:19.502771+00:00 | codex | REPAIRED_METHANOGEN_STOCK_DOSES_AND_BASE_REFERENCE_WITH_EVIDENCE | Corrected recipe-specific quantities and provenance; preserved stock-strength conflicts and unresolved final-volume/base-composition claims. Existing history, conditions and organism claims unchanged. |