Overview
- External ontology
- mediadive.medium:J98 (source link unavailable) — ALKALINE LACTOSE AGAR
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=98
Composition
flowchart LR
CultureMech_003339["alkaline_lactose_agar"]
ing0_Lactose["Lactose"]
CultureMech_003339 --> ing0_Lactose
CHEBI_17716["CHEBI:17716"]
ing0_Lactose -.-> CHEBI_17716
ing1_Yeast_extract["Yeast extract"]
CultureMech_003339 --> ing1_Yeast_extract
ing2_Polypeptone["Polypeptone"]
CultureMech_003339 --> ing2_Polypeptone
ing3_K2HPO4["K2HPO4"]
CultureMech_003339 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003339 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Agar["Agar"]
CultureMech_003339 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Na2CO3["Na2CO3"]
CultureMech_003339 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Lactose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17716
lactose
|
|
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 | Polypeptone | 5 G_PER_L | ||
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
100 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- Separately sterilize Na2CO3 solution by filtration, and aseptically add to the medium. Check final pH to be about 10.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.726719Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J98 |
| 2026-03-13T06:19:19.634785Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003339 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.668071+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALINE LACTOSE AGAR" to "alkaline_lactose_agar" for consistent matching |
| 2026-06-05T07:40:47.882390+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.801168+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |