Overview
- External ontology
- mediadive.medium:J997 (source link unavailable) — MARINITOGA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=997
Composition
flowchart LR
CultureMech_003346["marinitoga_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_003346 --> ing0_Sea_Salt
ing1_PIPES["PIPES"]
CultureMech_003346 --> ing1_PIPES
CHEBI_39033["CHEBI:39033"]
ing1_PIPES -.-> CHEBI_39033
ing2_Yeast_extract["Yeast extract"]
CultureMech_003346 --> ing2_Yeast_extract
ing3_Tryptone["Tryptone"]
CultureMech_003346 --> ing3_Tryptone
ing4_Glucose["Glucose"]
CultureMech_003346 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Resazurin["Resazurin"]
CultureMech_003346 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003346 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003346 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 30 G_PER_L | ||
| 2 | PIPES | 6 G_PER_L |
CHEBI:39033
PIPES
|
|
| 3 | Yeast extract | 1 G_PER_L | ||
| 4 | Tryptone | 1 G_PER_L | ||
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Preparation
- Dissolve ingredients except glucose and reducing agents. Prepare medium anaerobically under a 100% N2 gas-atmosphere, adjust pH to 7.0, dispense in anaerobic vials (1/3 vol.) and autoclave. Add glucose, cysteine and sulfide from sterile, anoxic stock solutions prepared under N2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.263614Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J997 |
| 2026-03-13T06:19:19.689839Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003346 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.729721+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MARINITOGA MEDIUM" to "marinitoga_medium" for consistent matching |
| 2026-06-05T07:40:17.976781+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |