Overview
- External ontology
- komodo.medium:1006 (source link unavailable) — ALLISONELLA medium
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: KOMODO ModelSEED | ID: 1006 | DSMZ Medium: 1006 (mediadive.medium:1006) | Aerobic: Yes Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. KOMODO reports Is Aerobic: true, while its linked DSMZ original uses 100% CO2 and anoxic vessels. Preserve this source conflict, not a resolved oxygen requirement or quantitative gas addition. The linked original specifies anoxic preparation; it does not establish a quantitative CO2 ingredient amount or justify replacing this provider's source-specific rates. Archive the complete prior ingredient nodes, including the copied L-cysteine and sodium-resazurin annotations, in append-only history. Their source-specific replacements do not inherit unverified chemical forms. Source-specific ingredient identities and quantity scopes supersede copied flattened amounts. Unknown gas amounts, densities and final volumes remain unknown. Other existing chemical annotations and all prior history are preserved, not recertified. No taxa-media association is added.
Composition
flowchart LR
CultureMech_003506["allisonella_medium"]
ing0_Na2CO3["Na2CO3"]
CultureMech_003506 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_iso_Valeric_acid["iso-Valeric acid"]
CultureMech_003506 --> ing1_iso_Valeric_acid
CHEBI_28484["CHEBI:28484"]
ing1_iso_Valeric_acid -.-> CHEBI_28484
ing2_DL_histidine["DL-histidine"]
CultureMech_003506 --> ing2_DL_histidine
CHEBI_27570["CHEBI:27570"]
ing2_DL_histidine -.-> CHEBI_27570
ing3_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_003506 --> ing3_Cysteine_HCl_x_H2O
ing4_Propionic_acid["Propionic acid"]
CultureMech_003506 --> ing4_Propionic_acid
CHEBI_30768["CHEBI:30768"]
ing4_Propionic_acid -.-> CHEBI_30768
ing5_CO2["CO2"]
CultureMech_003506 --> ing5_CO2
ing6_Distilled_water["Distilled water"]
CultureMech_003506 --> ing6_Distilled_water
ing7_DL_2_Methyl_butyric_acid["DL-2-Methyl butyric acid"]
CultureMech_003506 --> ing7_DL_2_Methyl_butyric_acid
ing8_K2HPO4["K2HPO4"]
CultureMech_003506 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_KH2PO4["KH2PO4"]
CultureMech_003506 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_iso_Butyric_acid["iso-Butyric acid"]
CultureMech_003506 --> ing10_iso_Butyric_acid
CHEBI_16135["CHEBI:16135"]
ing10_iso_Butyric_acid -.-> CHEBI_16135
ing11_n_Valeric_acid["n-Valeric acid"]
CultureMech_003506 --> ing11_n_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing11_n_Valeric_acid -.-> CHEBI_17418
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003506 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_Butyric_acid["Butyric acid"]
CultureMech_003506 --> ing13_Butyric_acid
CHEBI_30772["CHEBI:30772"]
ing13_Butyric_acid -.-> CHEBI_30772
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003506 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_Yeast_extract["Yeast extract"]
CultureMech_003506 --> ing15_Yeast_extract
ing16_Trypticase["Trypticase"]
CultureMech_003506 --> ing16_Trypticase
ing17_Resazurin["Resazurin"]
CultureMech_003506 --> ing17_Resazurin
ing18_NaCl["NaCl"]
CultureMech_003506 --> ing18_NaCl
CHEBI_26710["CHEBI:26710"]
ing18_NaCl -.-> CHEBI_26710
ing19_Acetic_acid["Acetic acid"]
CultureMech_003506 --> ing19_Acetic_acid
CHEBI_15366["CHEBI:15366"]
ing19_Acetic_acid -.-> CHEBI_15366
ing20__NH4_2SO4["(NH4)2SO4"]
CultureMech_003506 --> ing20__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing20__NH4_2SO4 -.-> CHEBI_62946
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
3.99 G_PER_L |
CHEBI:29377
sodium carbonate
|
Provider-reported medium rate: Gram Amount 3.99 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
| 2 |
iso-Valeric acid
C5H10O2 102.133 g/mol
|
CHEBI:28484
isovaleric acid
|
The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 3 |
DL-histidine
C6H9N3O2 155.157 g/mol
|
7.78 G_PER_L |
CHEBI:27570
histidine
|
Provider-reported medium rate: Gram Amount 7.78 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
| 4 | Cysteine-HCl x H2O | 0.50 G_PER_L | Provider-reported medium rate: Gram Amount 0.50 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. The source label does not explicitly reproduce the copied sodium-salt or L-stereochemistry identity. Withdraw the copied annotations rather than infer that form; archive the original node in history. This is not a claim that a particular alternative chemical form is proven. | |
| 5 |
Propionic acid
C3H6O2 74.079 g/mol
|
CHEBI:30768
propionic acid
|
The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 6 | CO2 | The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | ||
| 7 | Distilled water | The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | ||
| 8 | DL-2-Methyl butyric acid | The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | ||
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.29 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Provider-reported medium rate: Gram Amount 0.29 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.29 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Provider-reported medium rate: Gram Amount 0.29 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
| 11 |
iso-Butyric acid
C4H8O2 88.106 g/mol
|
CHEBI:16135
isobutyric acid
|
The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 12 |
n-Valeric acid
C5H10O2 102.133 g/mol
|
CHEBI:17418
valeric acid
|
The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Provider-reported medium rate: Gram Amount 0.10 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
| 14 |
Butyric acid
C4H8O2 88.106 g/mol
|
CHEBI:30772
butyric acid
|
The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.06 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Provider-reported medium rate: Gram Amount 0.06 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
| 16 | Yeast extract | 3.99 G_PER_L | Provider-reported medium rate: Gram Amount 3.99 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 17 | Trypticase | 1.00 G_PER_L | Provider-reported medium rate: Gram Amount 1.00 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 18 | Resazurin | 9.97E-4 G_PER_L | Provider-reported medium rate: Gram Amount 9.97E-4 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. The source label does not explicitly reproduce the copied sodium-salt or L-stereochemistry identity. Withdraw the copied annotations rather than infer that form; archive the original node in history. This is not a claim that a particular alternative chemical form is proven. | |
| 19 |
NaCl
Cl.Na 58.443 g/mol
|
0.48 G_PER_L |
CHEBI:26710
sodium chloride
|
Provider-reported medium rate: Gram Amount 0.48 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
| 20 |
Acetic acid
C2H4O2 60.052 g/mol
|
CHEBI:15366
acetic acid
|
The source Gram Amount is null: no fixed amount is asserted, and missing is not zero. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. | |
| 21 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.48 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Provider-reported medium rate: Gram Amount 0.48 per liter, preserving displayed precision. The linked DSMZ preparation does not independently explain this normalization. The Mol Amount and separate SEED aggregate table are not used to infer ingredient forms, stock doses or new molarities. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1006 |
| 2026-02-03T11:19:05.235270 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1006 |
| 2026-03-13T06:19:20.270791Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003506 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:34.379832+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALLISONELLA medium" to "allisonella_medium" for consistent matching |
| 2026-06-05T07:40:18.079524+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.237222+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T02:20:31.460987+00:00 | codex | RESTORED_ALLISONELLA_ACID_IDENTITIES_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore source-specific Allisonella ingredients and the acid/resazurin stock boundaries; retain unquantified amounts, chemical-form limits and source conflicts, and correct the reciprocal family relationship without changing permanent IDs. |