Overview
- External ontology
- komodo.medium:1007 (source link unavailable) — MINERAL MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.5, 'max': 6.0}
- Notes
- Source: KOMODO ModelSEED | ID: 1007 | DSMZ Medium: 1007 (mediadive.medium:1007) | Aerobic: No
Composition
flowchart LR
CultureMech_003510["mineral_medium"]
ing0_KNO3["KNO3"]
CultureMech_003510 --> ing0_KNO3
CHEBI_63043["CHEBI:63043"]
ing0_KNO3 -.-> CHEBI_63043
ing1_KH2PO4["KH2PO4"]
CultureMech_003510 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003510 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003510 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Distilled_water["Distilled water"]
CultureMech_003510 --> ing4_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing4_Distilled_water -.-> CHEBI_15377
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO3
K.NO3 101.102 g/mol
|
0.25 G_PER_L |
CHEBI:63043
potassium nitrate
|
DSMZ Medium 1007 lists 250.0 mg KNO3 in the main 1000 ml solution. |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
DSMZ Medium 1007 lists 100.0 mg KH2PO4 in the main 1000 ml solution. |
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
DSMZ Medium 1007 lists 50.0 mg MgSO4 x 7 H2O in the main 1000 ml solution. |
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.01 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
DSMZ Medium 1007 lists 10.0 mg CaCl2 x 2 H2O in the main 1000 ml solution. |
| 5 |
Distilled water
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
DSMZ Medium 1007 makes the main solution with 1000.0 ml distilled water. |
Preparation
- Adjust the final medium to pH 5.5-6.0.
- Use shaking incubation for strains grown on methane.
Solutions
Trace elements
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| EDTA | 5.00 G_PER_L | DSMZ Medium 1007 lists 5.00 g EDTA in the trace elements stock. |
| CuCl2 x 5 H2O | 0.10 G_PER_L | DSMZ Medium 1007 lists 0.10 g CuCl2 x 5 H2O in the trace elements stock. |
| FeSO4 x 7 H2O | 2.00 G_PER_L | DSMZ Medium 1007 lists 2.00 g FeSO4 x 7 H2O in the trace elements stock. |
| ZnSO4 x 7 H2O | 0.10 G_PER_L | DSMZ Medium 1007 lists 0.10 g ZnSO4 x 7 H2O in the trace elements stock. |
| NiCl2 x 6 H2O | 0.02 G_PER_L | DSMZ Medium 1007 lists 0.02 g NiCl2 x 6 H2O in the trace elements stock. |
| CoCl2 x 6 H2O | 0.20 G_PER_L | DSMZ Medium 1007 lists 0.20 g CoCl2 x 6 H2O in the trace elements stock. |
| Na2MoO4 | 0.03 G_PER_L | DSMZ Medium 1007 lists 0.03 g Na2MoO4 in the trace elements stock. |
| MnCl2 x 4 H2O | 0.03 G_PER_L | DSMZ Medium 1007 lists 0.03 g MnCl2 x 4 H2O in the trace elements stock. |
| Distilled water | 1000.00 ML_PER_L | DSMZ Medium 1007 makes the trace elements stock with 1000.00 ml distilled water. |
DSMZ Medium 1007 adds 1.0 ml/L trace elements stock.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1007 |
| 2026-02-03T11:19:19.991083 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1007 |
| 2026-03-13T06:19:20.296567Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003510 |
| 2026-03-17T06:30:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:34.409528+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MINERAL MEDIUM" to "mineral_medium" for consistent matching |
| 2026-06-05T07:40:18.090520+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.125090+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:43.242546+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-12T00:00:00-07:00 | repair_dsmz_1007_trace_stock_score15.py | RESOLVED_DSMZ_1007_TRACE_STOCK_SCORE15 | DSMZ Medium 1007 defines Mineral Medium with KNO3, KH2PO4, MgSO4 x 7 H2O, CaCl2 x 2 H2O, 1.0 ml/L trace elements, and distilled water at final pH 5.5-6.0. The trace elements stock contains EDTA, CuCl2 x 5 H2O, FeSO4 x 7 H2O, ZnSO4 x 7 H2O, NiCl2 x 6 H2O, CoCl2 x 6 H2O, Na2MoO4, MnCl2 x 4 H2O, and distilled water per liter. |