Overview
- External ontology
- komodo.medium:1008 (source link unavailable) — MARINE SPIROCHETE medium
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- pH buffer: KOH | Source: KOMODO ModelSEED | ID: 1008 | DSMZ Medium: 1008 (mediadive.medium:1008) | Aerobic: No
Composition
flowchart LR
CultureMech_003513["marine_spirochete_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_003513 --> ing0_Trypticase_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_003513 --> ing1_Yeast_extract
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_003513 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
ing3_Na_thioglycolate["Na-thioglycolate"]
CultureMech_003513 --> ing3_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing3_Na_thioglycolate -.-> CHEBI_86481
ing4_Cellobiose["Cellobiose"]
CultureMech_003513 --> ing4_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing4_Cellobiose -.-> CHEBI_17057
ing5_Charcoal_filtered__natural_seawater["Charcoal-filtered, natural seawater"]
CultureMech_003513 --> ing5_Charcoal_filtered__natural_seawater
ing6_KOH["KOH"]
CultureMech_003513 --> ing6_KOH
CHEBI_32035["CHEBI:32035"]
ing6_KOH -.-> CHEBI_32035
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 2 G_PER_L | ||
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
1 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 5 |
Cellobiose
C12H22O11 342.297 g/mol
|
2 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 6 | Charcoal-filtered, natural seawater | 800 G_PER_L | ||
| 7 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1008 |
| 2026-02-03T11:19:00.282816 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1008 |
| 2026-03-13T03:08:52.437636Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: KOH |
| 2026-03-13T06:19:20.308298Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003513 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:34.422462+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MARINE SPIROCHETE medium" to "marine_spirochete_medium" for consistent matching |
| 2026-05-16T09:03:40.398749+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:13.038786+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.938477+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |