for_dsm_15769

CultureMech:003515 bacterial DEFINED LIQUID
Original name: For DSM 15769

Overview

External ontology
komodo.medium:1010.1 (source link unavailable) — For DSM 15769
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.5}
Notes
Source: KOMODO ModelSEED | ID: 1010.1 | DSMZ Medium: 1010 (mediadive.medium:1010) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_003515["for_dsm_15769"]
    ing0_NaCl["NaCl"]
    CultureMech_003515 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003515 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003515 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_003515 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003515 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_KBr["KBr"]
    CultureMech_003515 --> ing5_KBr
    CHEBI_32030["CHEBI:32030"]
    ing5_KBr -.-> CHEBI_32030
    ing6_Sodium_resazurin["Sodium resazurin"]
    CultureMech_003515 --> ing6_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Sodium_resazurin -.-> CHEBI_8806
    ing7_KH2PO4["KH2PO4"]
    CultureMech_003515 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_NH4Cl["NH4Cl"]
    CultureMech_003515 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_Na2CO3["Na2CO3"]
    CultureMech_003515 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Na_DL_lactate["Na-DL-lactate"]
    CultureMech_003515 --> ing10_Na_DL_lactate
    CHEBI_75228["CHEBI:75228"]
    ing10_Na_DL_lactate -.-> CHEBI_75228
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003515 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_HCl["HCl"]
    CultureMech_003515 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_ZnCl2["ZnCl2"]
    CultureMech_003515 --> ing13_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing13_ZnCl2 -.-> CHEBI_49976
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003515 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_003515 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003515 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003515 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003515 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003515 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_NaOH["NaOH"]
    CultureMech_003515 --> ing20_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing20_NaOH -.-> CHEBI_32145
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003515 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003515 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing23_Biotin["Biotin"]
    CultureMech_003515 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_003515 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003515 --> ing25_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing25_Thiamine_HCl -.-> CHEBI_49105
    ing26_Riboflavin["Riboflavin"]
    CultureMech_003515 --> ing26_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing26_Riboflavin -.-> CHEBI_17015
    ing27_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_003515 --> ing27_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing28__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_003515 --> ing28__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing29_D_____biotin["D-(+)-biotin"]
    CultureMech_003515 --> ing29_D_____biotin
    ing29_D_____biotin -.-> CHEBI_15956
    CultureMech_003515 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
26.2948 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.67729 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.77291 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.657371 G_PER_L CHEBI:32588
potassium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.46414 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 KBr
Br.K 119.002 g/mol
0.0896414 G_PER_L CHEBI:32030
potassium bromide
7 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000498008 G_PER_L CHEBI:8806
Resazurin
8 KH2PO4
H2O4P.K 136.084 g/mol
0.199203 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 NH4Cl
Cl.H4N 53.492 g/mol
0.249004 G_PER_L CHEBI:31206
ammonium chloride
10 Na2CO3
CO3.2Na 105.988 g/mol
0.996016 G_PER_L CHEBI:29377
sodium carbonate
11 Na-DL-lactate
C3H5O3.Na 112.06 g/mol
2.29084 G_PER_L CHEBI:75228
sodium lactate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.298805 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
14 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
25 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
26 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
27 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
28 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
29 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
30 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
31 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Solutions

Trace element solution SL-10

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.5 G_PER_L

Seven vitamins solution

Stock composition
IngredientConcentrationNotes
Thiamine-HCl x 2 H2O 0.2 G_PER_L
Nicotinic acid 0.2 G_PER_L [Merged 2 duplicates: 0.05, 0.2]
p-Aminobenzoic acid 0.08 G_PER_L [Merged 2 duplicates: 0.05, 0.08]
Pyridoxine hydrochloride 0.3 G_PER_L [Merged 2 duplicates: 0.1, 0.3]
Vitamin B12 0.1 G_PER_L [Merged 2 duplicates: 0.001, 0.1]

Wolin's vitamin solution (10x)

Stock composition
IngredientConcentrationNotes
Nicotinic acid 0.05 G_PER_L [Merged 2 duplicates: 0.05, 0.2]
p-Aminobenzoic acid 0.05 G_PER_L [Merged 2 duplicates: 0.05, 0.08]
Pyridoxine hydrochloride 0.1 G_PER_L [Merged 2 duplicates: 0.1, 0.3]
Vitamin B12 0.001 G_PER_L [Merged 2 duplicates: 0.001, 0.1]

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:01.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 1010.1
2026-02-03T11:19:06.551146 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 1010
2026-02-06T05:10:32.729687Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:09.819819Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:20.323583Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003515
2026-03-17T06:30:16.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:34.438851+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "For DSM 15769" to "for_dsm_15769" for consistent matching
2026-05-20T06:01:18.124078+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.5' -> {'min': 7.2, 'max': 7.5}
2026-06-05T07:41:02.212410+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:57.548622+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-15T00:17:29.992014+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 6 stock-strength component(s) out of ingredients into 3 solution(s): 1 -> 'Trace element solution SL-10' @ 1 ml/l; 1 -> 'Seven vitamins solution' @ 1 ml/l; 0 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. 4 component(s) were SUMMED across stocks by the flattening and are restored to each stock's own value, verified by exact sum: nicotinic acid, p-aminobenzoic acid, pyridoxine hydrochloride, vitamin b12. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).
2026-09-13T00:00:00-07:00 repair_komodo_1010_artificial_seawater_score10.py RESOLVED_KOMODO_1010_ARTIFICIAL_SEAWATER_STRAIN_VARIANT KOMODO Medium 1010.1 preserves ARTIFICIAL SEAWATER MEDIUM components and pH 7.2-7.5 but applies the recipe for DSM 15769.