medium_1021_modified_for_dsm_16929

Overview

External ontology

komodo.medium:1021_16929 (source link unavailable) — MEDIUM 1021 MODIFIED FOR DSM 16929

Applications

• Microbial cultivation

Notes

Source: KOMODO ModelSEED | ID: 1021_16929 | DSMZ Medium: 1021 (mediadive.medium:1021) | Aerobic: No Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The false LB constituent expansion is withdrawn. Each source's actual hydrolysate and preparation scope is retained. Existing unrelated chemical annotations and history are preserved, not recertified; no taxa-media growth association is added. Withdraw the copied pH 8.0 assertion: this variant's own page has no pH value. The linked protocol's Solution A adjustment is not a final variant pH measurement.

Composition

flowchart LR
    CultureMech_003533["medium_1021_modified_for_dsm_16929"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003533 --> ing0_Yeast_extract
    ing1_D_Glucose["D-Glucose"]
    CultureMech_003533 --> ing1_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing1_D_Glucose -.-> CHEBI_17634
    ing2_KCl["KCl"]
    CultureMech_003533 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003533 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003533 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_003533 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_NaHCO3["NaHCO3"]
    CultureMech_003533 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Agar["Agar"]
    CultureMech_003533 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_003533 --> ing8_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing8_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing9_casein_hydrolysate["casein hydrolysate"]
    CultureMech_003533 --> ing9_casein_hydrolysate
    ing10_Distilled_water["Distilled water"]
    CultureMech_003533 --> ing10_Distilled_water
    ing11_new_borne_calf_serum["new borne calf serum"]
    CultureMech_003533 --> ing11_new_borne_calf_serum
    ing12_O2["O2"]
    CultureMech_003533 --> ing12_O2
    ing13_N2["N2"]
    CultureMech_003533 --> ing13_N2
    ing14_CO2["CO2"]
    CultureMech_003533 --> ing14_CO2

Ingredients (15)

Preparation

1. Prepare Solution A according to the linked DSMZ 1021 protocol and adjust Solution A to pH 8.0. This is not a measured final-medium pH.
2. For the solid preparation, sterilize Solution A and Solution B separately at 121 C for 20 min. Do not autoclave the serum-supplemented mixture.
3. Cool the separately sterilized solutions to 50 C, then combine A and B.
4. To 4 parts of the combined basal preparation, add 1 part sterile foetal or newborn calf serum. Preserve the reported ratio; no fixed serum batch dose or mass concentration is asserted.

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  Liquid preparation described by DSMZ 1021

  Relationship

  PHYSICAL_STATE_VARIANT

  Description

  The source describes an alternative liquid preparation but does not provide a separate complete ingredient table.

  Modifications

  • Filter sterilize the liquid preparation instead of autoclaving. No membrane pore size is specified.
  • The source does not explicitly quantify the agar change for this alternative; no numeric agar amount is invented.

  Evidence

  • Reference

    https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1021.pdf

    Supports

    SUPPORT

    Snippet

    Sterilize solution A and B separately at 121C for 20 min. Cool to 50C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved.

    Explanation

    variants[0].modifications: Preserve the source's conditional liquid preparation, not an invented fully quantified broth recipe. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-08T23:33:46.995198+00:00; capture SHA256=1326bfc6d79f8ed3ad765909aa48d3e4efbb378516ada4a4087710860d8b7cc8.

Provenance