manning_medium (CultureMech:003535)

Overview

External ontology

komodo.medium:1023 (source link unavailable) — MANNING medium

Applications

Microbial cultivation

pH range

{'min': 2.5, 'max': 2.7}

Notes

pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 1023 | DSMZ Medium: 1023 (mediadive.medium:1023) | Aerobic: No

Composition

flowchart LR
    CultureMech_003535["manning_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003535 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KCl["KCl"]
    CultureMech_003535 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_K2HPO4["K2HPO4"]
    CultureMech_003535 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003535 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_003535 --> ing4_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing4_Ca_NO3_2 -.-> CHEBI_64205
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003535 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_003535 --> ing6_Yeast_extract
    ing7_H2SO4["H2SO4"]
    CultureMech_003535 --> ing7_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing7_H2SO4 -.-> CHEBI_26836

Ingredients (8)

#	Ingredient	Concentration	Ontology	Notes
1	(NH4)2SO4 2H4N.O4S 132.141 g/mol	6 G_PER_L	CHEBI:62946 ammonium sulfate
2	KCl Cl.K 74.551 g/mol	0.2 G_PER_L	CHEBI:32588 potassium chloride
3	K2HPO4 HO4P.2K 174.174 g/mol	0.2 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	1 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
5	Ca(NO3)2 Ca.2NO3 164.086 g/mol	0.02 G_PER_L	CHEBI:64205 calcium nitrate
6	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	33.4 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
7	Yeast extract	0.2 G_PER_L
8	H2SO4 H2O4S 98.079 g/mol	variable VARIABLE	CHEBI:26836 sulfuric acid	pH adjustment (extracted from notes)

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:01.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 1023
2026-02-03T11:19:16.458569	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 1023
2026-02-06T05:10:49.437512Z	invalid-chebi-removal	Removed 1 invalid CHEBI ID(s)	Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:35.476467Z	data-quality-cleanup-v1.0	Data quality fixes	Added pH buffer: H2SO4
2026-03-13T06:19:20.495742Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:003535
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: H2SO4
2026-03-17T06:30:46.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:34.639258+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MANNING medium" to "manning_medium" for consistent matching
2026-05-16T09:02:59.643669+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-05-20T06:00:48.369535+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '2.5-2.7' -> {'min': 2.5, 'max': 2.7}
2026-06-05T07:40:18.187281+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.485651+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:43.256261+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.