Overview
- External ontology
- komodo.medium:1078 (source link unavailable) — FRIIS medium
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: KOMODO ModelSEED | ID: 1078 | DSMZ Medium: 1078 (mediadive.medium:1078) | Aerobic: No Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_003730["friis_medium"]
ing0_Calf_brains["Calf brains"]
CultureMech_003730 --> ing0_Calf_brains
ing1_Beef_heart["Beef heart"]
CultureMech_003730 --> ing1_Beef_heart
ing2_Proteose_peptone["Proteose peptone"]
CultureMech_003730 --> ing2_Proteose_peptone
ing3_Dextrose["Dextrose"]
CultureMech_003730 --> ing3_Dextrose
CHEBI_17634["CHEBI:17634"]
ing3_Dextrose -.-> CHEBI_17634
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_003730 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Disodium_phosphate["Disodium phosphate"]
CultureMech_003730 --> ing5_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Disodium_phosphate -.-> CHEBI_34683
ing6_PPLO_broth["PPLO broth"]
CultureMech_003730 --> ing6_PPLO_broth
ing7_Porcine_serum["Porcine serum"]
CultureMech_003730 --> ing7_Porcine_serum
ing8_Yeast_extract["Yeast extract"]
CultureMech_003730 --> ing8_Yeast_extract
ing9_Phenol_red["Phenol red"]
CultureMech_003730 --> ing9_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing9_Phenol_red -.-> CHEBI_31991
ing10_NaCl["NaCl"]
CultureMech_003730 --> ing10_NaCl
ing10_NaCl -.-> CHEBI_26710
ing11_KCl["KCl"]
CultureMech_003730 --> ing11_KCl
CHEBI_32588["CHEBI:32588"]
ing11_KCl -.-> CHEBI_32588
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003730 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003730 --> ing13_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing13_MgCl2_x_6_H2O -.-> CHEBI_86345
ing14_CaCl2["CaCl2"]
CultureMech_003730 --> ing14_CaCl2
CHEBI_3312["CHEBI:3312"]
ing14_CaCl2 -.-> CHEBI_3312
ing15_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_003730 --> ing15_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing15_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing16_KH2PO4["KH2PO4"]
CultureMech_003730 --> ing16_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing16_KH2PO4 -.-> CHEBI_63036
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 2 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 4 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 6 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 7 | PPLO broth | 5.40373 G_PER_L | ||
| 8 | Porcine serum | 30 G_PER_L | ||
| 9 | Yeast extract | 0.83 G_PER_L | ||
| 10 |
Phenol red
C19H14O5S 354.383 g/mol
|
0.00133 G_PER_L |
CHEBI:31991
phenol red
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
160 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 12 |
KCl
Cl.K 74.551 g/mol
|
8 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 15 |
CaCl2
Ca.2Cl 110.984 g/mol
|
3.7 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 16 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
3 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 17 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- Mycoplasma hyopneumoniae
- Term
- Id
- NCBITaxon:2099
- Label
- Mesomycoplasma hyopneumoniae
- Strain
- F1.12A; F5.6A; F6.12D; F7.2C; F12.6A; 13.7B; Mp143
- Growth metrics
- Doubling time minutes
- 288.0
- Measurement conditions
- Modified Friis medium; seven M. hyopneumoniae isolates in slowly shaken cultures; source reports doubling times ranging from 4.8 to 7.8 h, with this value recording the lower bound.
- Evidence
- Reference
- PMID:20851152
- Supports
- SUPPORT
- Snippet
- Doubling times (Tds) of the isolates grown in slowly shaken cultures varied between 4.8 and 7.8 h.
- Explanation
- Manual literature review found direct growth-kinetics evidence for seven named Mycoplasma hyopneumoniae isolates in modified Friis medium.
- Is max attainment
- False
Recipe variants
- Name
- friis_medium_mycoplasma_hyopneumoniae_isolate_growth
- Description
- Modified Friis medium condition supporting growth of seven Mycoplasma hyopneumoniae isolates measured by ATP luminometry.
- Modifications
- Use modified Friis medium as described by Friis 1975.
- Incubate slowly shaken Mycoplasma hyopneumoniae cultures; source reports isolate-dependent behavior across five Friis medium batches.
- Purpose
- Capture isolate-level Mycoplasma hyopneumoniae growth evidence under the parent Friis medium record.
- Evidence
- Reference
- PMID:20851152
- Supports
- SUPPORT
- Snippet
- Doubling times (Tds) of the isolates grown in slowly shaken cultures varied between 4.8 and 7.8 h.
- Explanation
- The source directly measured growth kinetics for seven named Mycoplasma hyopneumoniae isolates in modified Friis medium, supporting this condition as a variant of the Friis parent medium.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1078 |
| 2026-02-03T11:19:11.121373 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1078 |
| 2026-03-13T06:19:21.743179Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003730 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:36.019533+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FRIIS medium" to "friis_medium" for consistent matching |
| 2026-05-05T00:00:00+00:00 | literature_review_manual | ADDED_GROWTH_EVIDENCE | Added Mycoplasma hyopneumoniae isolate growth evidence from PMID:20851152 as a Friis medium variant. |
| 2026-06-05T07:40:18.761300+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.409658+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T00:58:02.606017+00:00 | curate_organism_culture_type.py | SET_ORGANISM_CULTURE_TYPE | Inferred isolate: 1 specific strain(s) named, no community signal (#142) |