Overview
- External ontology
- komodo.medium:110_3994 (source link unavailable) — MEDIUM 110 MODIFIED FOR DSM 3994
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 110_3994 | DSMZ Medium: 110 (mediadive.medium:110) | Aerobic: No
Composition
flowchart LR
CultureMech_003797["medium_110_modified_for_dsm_3994"]
ing0_Ground_beef["Ground beef"]
CultureMech_003797 --> ing0_Ground_beef
ing1_NaOH["NaOH"]
CultureMech_003797 --> ing1_NaOH
CHEBI_32145["CHEBI:32145"]
ing1_NaOH -.-> CHEBI_32145
ing2_Casitone["Casitone"]
CultureMech_003797 --> ing2_Casitone
ing3_Yeast_extract["Yeast extract"]
CultureMech_003797 --> ing3_Yeast_extract
ing4_K2HPO4["K2HPO4"]
CultureMech_003797 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_003797 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_D_Glucose["D-Glucose"]
CultureMech_003797 --> ing6_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing6_D_Glucose -.-> CHEBI_17634
ing7_Cellobiose["Cellobiose"]
CultureMech_003797 --> ing7_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing7_Cellobiose -.-> CHEBI_17057
ing8_Maltose["Maltose"]
CultureMech_003797 --> ing8_Maltose
CHEBI_17306["CHEBI:17306"]
ing8_Maltose -.-> CHEBI_17306
ing9_Starch["Starch"]
CultureMech_003797 --> ing9_Starch
CHEBI_28017["CHEBI:28017"]
ing9_Starch -.-> CHEBI_28017
ing10_L_Cysteine_HCl["L-Cysteine HCl"]
CultureMech_003797 --> ing10_L_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing10_L_Cysteine_HCl -.-> CHEBI_91247
ing11_Agar["Agar"]
CultureMech_003797 --> ing11_Agar
CHEBI_2509["CHEBI:2509"]
ing11_Agar -.-> CHEBI_2509
ing12_Haemin["Haemin"]
CultureMech_003797 --> ing12_Haemin
CHEBI_50385["CHEBI:50385"]
ing12_Haemin -.-> CHEBI_50385
ing13_Vitamin_K1["Vitamin K1"]
CultureMech_003797 --> ing13_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing13_Vitamin_K1 -.-> CHEBI_18067
ing14_Ethanol["Ethanol"]
CultureMech_003797 --> ing14_Ethanol
CHEBI_16236["CHEBI:16236"]
ing14_Ethanol -.-> CHEBI_16236
ing15_Vitamin_K3["Vitamin K3"]
CultureMech_003797 --> ing15_Vitamin_K3
CHEBI_28869["CHEBI:28869"]
ing15_Vitamin_K3 -.-> CHEBI_28869
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Ground beef | 500 G_PER_L | ||
| 2 |
NaOH
HO.Na 39.997 g/mol
|
26.0 G_PER_L |
CHEBI:32145
sodium hydroxide
|
[Merged 2 duplicates: 25.0, 1.0] |
| 3 | Casitone | 30 G_PER_L | ||
| 4 | Yeast extract | 5 G_PER_L | ||
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
D-Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 8 |
Cellobiose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 9 |
Maltose
C12H22O11 342.297 g/mol
|
1 G_PER_L |
CHEBI:17306
maltose
|
|
| 10 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
|
| 11 |
L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.5 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
(add to make medium anoxic) |
| 12 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if required) |
| 13 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.5 G_PER_L |
CHEBI:50385
hemin
|
|
| 14 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.1 G_PER_L |
CHEBI:18067
phylloquinone
|
|
| 15 |
Ethanol
C2H6O 46.069 g/mol
|
959.5 G_PER_L |
CHEBI:16236
ethanol
|
[Merged 2 duplicates: 950.0, 9.5] |
| 16 |
Vitamin K3
C11H8O2 172.183 g/mol
|
0.05 G_PER_L |
CHEBI:28869
menadione
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 110_3994 |
| 2026-02-03T11:18:59.670409 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 110 |
| 2026-03-13T03:08:50.991695Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:22.282913Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003797 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:36.646219+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 110 MODIFIED FOR DSM 3994" to "medium_110_modified_for_dsm_3994" for consistent matching |
| 2026-06-05T07:41:14.434253+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.323983+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |