beijerinckia_medium

CultureMech:003822 bacterial DEFINED SOLID_AGAR
Original name: BEIJERINCKIA MEDIUM

Overview

External ontology
komodo.medium:111 (source link unavailable) — BEIJERINCKIA MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: KOMODO ModelSEED | ID: 111 | DSMZ Medium: 111 (mediadive.medium:111) | Aerobic: No Preparation scope: basal input is 950 ml distilled water plus the listed salts and agar; glucose (10 g in 50 ml H2O) is sterilized separately and mixed after cooling. The retained basal g/L values are provider-reported normalizations, not final-medium concentrations. The two water inputs do not establish a measured 1 L final volume. Historical import annotations and ontology mappings are retained, not revalidated.

Composition

flowchart LR
    CultureMech_003822["beijerinckia_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_003822 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003822 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003822 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003822 --> ing3_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing4_MnSO4_x_6_H2O["MnSO4 x 6 H2O"]
    CultureMech_003822 --> ing4_MnSO4_x_6_H2O
    CHEBI_131523["CHEBI:131523"]
    ing4_MnSO4_x_6_H2O -.-> CHEBI_131523
    ing5_ZnSO4_x_6_H2O["ZnSO4 x 6 H2O"]
    CultureMech_003822 --> ing5_ZnSO4_x_6_H2O
    CHEBI_132762["CHEBI:132762"]
    ing5_ZnSO4_x_6_H2O -.-> CHEBI_132762
    ing6_CuSO4_x_6_H2O["CuSO4 x 6 H2O"]
    CultureMech_003822 --> ing6_CuSO4_x_6_H2O
    CHEBI_91246["CHEBI:91246"]
    ing6_CuSO4_x_6_H2O -.-> CHEBI_91246
    ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003822 --> ing7_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing8_Agar["Agar"]
    CultureMech_003822 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Distilled_water["Distilled water"]
    CultureMech_003822 --> ing9_Distilled_water

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.842105 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Source batch input: 0.8 g. The retained 0.842105 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
2 KH2PO4
H2O4P.K 136.084 g/mol
0.210526 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Source batch input: 0.2 g. The retained 0.210526 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.105263 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Source batch input: 0.1 g. The retained 0.105263 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
4 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.0210526 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Source batch input: 20 mg. The retained 0.0210526 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
5 MnSO4 x 6 H2O
6H2O.Mn.O4S 259.091 g/mol
0.00210526 G_PER_L CHEBI:131523
manganese(II) sulfate hexahydrate
 Source batch input: 2 mg. The retained 0.00210526 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
6 ZnSO4 x 6 H2O
6H2O.O4S.Zn 269.543 g/mol
0.00526316 G_PER_L CHEBI:132762
zinc sulfate hexahydrate
 Source batch input: 5 mg. The retained 0.00526316 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
7 CuSO4 x 6 H2O
Cu.6H2O.O4S 267.699 g/mol
0.00421053 G_PER_L CHEBI:91246
copper(II) sulfate hexahydrate
 Source batch input: 4 mg. The retained 0.00421053 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
8 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.00526316 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Source batch input: 5 mg. The retained 0.00526316 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
9 Agar 15.7895 G_PER_L CHEBI:2509
agar
 Source batch input: 15 g. The retained 15.7895 g/L is the provider-declared rate normalized to its 950 ml main-preparation field, not an independently verified concentration after adding the separately prepared glucose. No final volume is inferred.
10 Distilled water 0.95 L mediadive.compound:4 (source link unavailable)
Distilled water
 950 ml distilled-water input per basal batch, not a final-volume makeup instruction.

Preparation

  1. Adjust pH to 6.5. Sterilize glucose separately (10 g in 50 ml H2O) and mix after cooling.

Solutions

Separately sterilized glucose preparation

Stock composition
IngredientConcentrationNotes
Glucose Source batch input: 10 g glucose in 50 ml H2O, sterilized separately and mixed after cooling. The former 10.5263 g/L divides by the unrelated 950 ml basal-water input and is withdrawn. No 200 g/L stock strength or 10 g/L final-medium concentration is asserted: neither final volume is specified.
Water 0.05 L 50 ml H2O input for the separately sterilized glucose; water purity and final stock volume are not specified.
Mix the separately prepared glucose with the cooled basal preparation. The 50 ml is water input, not a measured stock volume or a per-liter dose; no numeric stock addition concentration is asserted.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:01.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 111
2026-02-03T11:19:04.736112 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 111
2026-03-13T06:19:22.518537Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003822
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:36.918966+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BEIJERINCKIA MEDIUM" to "beijerinckia_medium" for consistent matching
2026-06-05T07:40:19.139827+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.544436+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T21:37:12.543274+00:00 codex RESTORED_DSMZ111_GLUCOSE_STOCK_AND_INPUT_BASES Restored separate glucose preparation, withdrew its wrongly staged 10.5263 g/L slot, and distinguished water inputs from final volumes. Preserved all previous history, source snippets, identifiers, mappings and variant links. Basal provider rates remain explicitly scoped; no growth association added.