Overview
- External ontology
- komodo.medium:119_11995 (source link unavailable) — MEDIUM 119 MODIFIED FOR DSM 11995
- Applications
-
- Microbial cultivation
- Notes
- Source: KOMODO ModelSEED | ID: 119_11995 | DSMZ Medium: 119 (mediadive.medium:119) | Aerobic: No Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The linked original uses anoxic preparation; its individual stock inputs and strain instructions must not silently replace this provider's reported medium rates. Linked original instruction (For DSM 1093, DSM 1125, DSM 11977, DSM 11978, DSM 11995, DSM 16643): Supplement medium after autoclaving with 1 ml/l Wolin's vitamin solution (10x, see medium 120), 0.30 g/l DL-dithiothreitol, and 0.15 g/l coenzyme M (2-mercaptoethanesulfonic acid) from anoxic stock solutions sterilized by filtration. Omit Na2S x 9 H2O and L-Cysteine HCl x H2O. This is a separate source/strain prescription, not an overwrite of the KOMODO table or new growth evidence. Any disagreement remains explicit. Archive all prior ingredient nodes, including false LB expansion, combined yeast amounts and copied or chemically inexact annotations, in append-only history. New nodes follow this exact source's stage and label; other unchanged chemical annotations are preserved, not recertified. Source-scoped recipe inputs supersede unsupported flattened concentrations and false LB identities. All prior history and permanent IDs remain unchanged; unquantified quantities remain unknown. No taxa-media association is added.
Composition
flowchart LR
CultureMech_003915["medium_119_modified_for_dsm_11995"]
ing0_NaHCO3["NaHCO3"]
CultureMech_003915 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_sludge_from_an_anaerobic_digester["sludge from an anaerobic digester"]
CultureMech_003915 --> ing1_sludge_from_an_anaerobic_digester
ing2_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003915 --> ing2_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing2_Na2S_x_9_H2O -.-> CHEBI_76209
ing3_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_003915 --> ing3_Cysteine_HCl_x_H2O
ing4_Isovaleric_acid["Isovaleric acid"]
CultureMech_003915 --> ing4_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing4_Isovaleric_acid -.-> CHEBI_28484
ing5_CO2["CO2"]
CultureMech_003915 --> ing5_CO2
ing6_NaOH["NaOH"]
CultureMech_003915 --> ing6_NaOH
CHEBI_32145["CHEBI:32145"]
ing6_NaOH -.-> CHEBI_32145
ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003915 --> ing7_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
ing8_KH2PO4["KH2PO4"]
CultureMech_003915 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003915 --> ing9_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003915 --> ing10_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003915 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_HCl["HCl"]
CultureMech_003915 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_ZnCl2["ZnCl2"]
CultureMech_003915 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003915 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_Yeast_extract["Yeast extract"]
CultureMech_003915 --> ing15_Yeast_extract
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003915 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_Na_formate["Na-formate"]
CultureMech_003915 --> ing17_Na_formate
CHEBI_62965["CHEBI:62965"]
ing17_Na_formate -.-> CHEBI_62965
ing18_Resazurin["Resazurin"]
CultureMech_003915 --> ing18_Resazurin
ing19_H2O["H2O"]
CultureMech_003915 --> ing19_H2O
ing20_2_Methylbutyric_acid["2-Methylbutyric acid"]
CultureMech_003915 --> ing20_2_Methylbutyric_acid
ing21_Distilled_water["Distilled water"]
CultureMech_003915 --> ing21_Distilled_water
ing22_H2["H2"]
CultureMech_003915 --> ing22_H2
ing23_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003915 --> ing23_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing23_FeSO4_x_7_H2O -.-> CHEBI_75836
ing24_NH4Cl["NH4Cl"]
CultureMech_003915 --> ing24_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing24_NH4Cl -.-> CHEBI_31206
ing25_Isobutyric_acid["Isobutyric acid"]
CultureMech_003915 --> ing25_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing25_Isobutyric_acid -.-> CHEBI_16135
ing26_Valeric_acid["Valeric acid"]
CultureMech_003915 --> ing26_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing26_Valeric_acid -.-> CHEBI_17418
ing27_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003915 --> ing27_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing27_CaCl2_x_2_H2O -.-> CHEBI_86158
ing28_Na_acetate["Na-acetate"]
CultureMech_003915 --> ing28_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing28_Na_acetate -.-> CHEBI_32954
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003915 --> ing29_NiCl2_x_6_H2O
CultureMech_003915 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.96 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Provider-reported rate: 3.96 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 2 | sludge from an anaerobic digester | 49.46 G_PER_L | Provider-reported rate: 49.46 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. Copied annotations do not establish the source's exact chemical form; withdraw them, preserve the literal source name, and archive the prior node. No replacement ontology identifier is inferred. | |
| 3 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Provider-reported rate: 0.49 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 4 | Cysteine-HCl x H2O | 0.49 G_PER_L | Provider-reported rate: 0.49 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. Copied annotations do not establish the source's exact chemical form; withdraw them, preserve the literal source name, and archive the prior node. No replacement ontology identifier is inferred. | |
| 5 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
0.49 G_PER_L |
CHEBI:28484
isovaleric acid
|
Provider-reported rate: 0.49 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 6 | CO2 | Provider Gram Amount is null; no fixed amount is asserted and missing is not zero. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. | ||
| 7 |
NaOH
HO.Na 39.997 g/mol
|
CHEBI:32145
sodium hydroxide
|
Provider Gram Amount is null; no fixed amount is asserted and missing is not zero. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. | |
| 8 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
1.87E-4 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Provider-reported rate: 1.87E-4 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.49 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Provider-reported rate: 0.49 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 10 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
1.97E-6 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Provider-reported rate: 1.97E-6 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 11 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.48E-3 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Provider-reported rate: 1.48E-3 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.40 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Provider-reported rate: 0.40 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 13 |
HCl
HCl 36.461 g/mol
|
6.65E-4 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Provider-reported rate: 6.65E-4 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
6.91E-5 G_PER_L |
CHEBI:49976
zinc dichloride
|
Provider-reported rate: 6.91E-5 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
9.87E-5 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Provider-reported rate: 9.87E-5 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 16 | Yeast extract | 1.19 G_PER_L | Superseded flattening history, not a current amount: [Merged 2 duplicates: 0.996016, 4.0] Provider-reported rate: 1.19 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. | |
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3.55E-5 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Provider-reported rate: 3.55E-5 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 18 |
Na-formate
CHO2.Na 68.007 g/mol
|
1.98 G_PER_L |
CHEBI:62965
sodium formate
|
Provider-reported rate: 1.98 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 19 | Resazurin | 9.89E-4 G_PER_L | Provider-reported rate: 9.89E-4 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. Copied annotations do not establish the source's exact chemical form; withdraw them, preserve the literal source name, and archive the prior node. No replacement ontology identifier is inferred. | |
| 20 | H2O | Provider Gram Amount is null; no fixed amount is asserted and missing is not zero. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. | ||
| 21 | 2-Methylbutyric acid | 0.49 G_PER_L | Provider-reported rate: 0.49 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. | |
| 22 | Distilled water | Provider Gram Amount is null; no fixed amount is asserted and missing is not zero. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. | ||
| 23 | H2 | Provider Gram Amount is null; no fixed amount is asserted and missing is not zero. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. | ||
| 24 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.98E-3 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Provider-reported rate: 1.98E-3 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 25 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.40 G_PER_L |
CHEBI:31206
ammonium chloride
|
Provider-reported rate: 0.40 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 26 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.49 G_PER_L |
CHEBI:16135
isobutyric acid
|
Provider-reported rate: 0.49 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 27 |
Valeric acid
C5H10O2 102.133 g/mol
|
0.49 G_PER_L |
CHEBI:17418
valeric acid
|
Provider-reported rate: 0.49 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 28 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Provider-reported rate: 0.05 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 29 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.99 G_PER_L |
CHEBI:32954
sodium acetate
|
Provider-reported rate: 0.99 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 30 | NiCl2 x 6 H2O | 2.37E-5 G_PER_L | Provider-reported rate: 2.37E-5 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. Copied annotations do not establish the source's exact chemical form; withdraw them, preserve the literal source name, and archive the prior node. No replacement ontology identifier is inferred. | |
| 31 |
H3BO3
H3BO3 61.833 g/mol
|
5.92E-6 G_PER_L |
CHEBI:33118
boric acid
|
Provider-reported rate: 5.92E-6 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
| 32 |
NaCl
Cl.Na 58.443 g/mol
|
0.40 G_PER_L |
CHEBI:26710
sodium chloride
|
Provider-reported rate: 0.40 g/L, preserving the displayed precision. This table reports medium rates, not stock inputs or stock addition volumes. No Mol Amount, SEED aggregate identity, density or linked DSMZ normalization is adopted. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 119_11995 |
| 2026-02-03T11:18:55.640886 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 119 |
| 2026-03-13T03:08:41.270484Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:23.229464Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003915 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:37.747673+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 119 MODIFIED FOR DSM 11995" to "medium_119_modified_for_dsm_11995" for consistent matching |
| 2026-06-05T07:41:14.571067+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.370371+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T03:06:56.858381+00:00 | codex | RESTORED_MEDIUM_119_SOURCE_FORMULATIONS_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore exact-source medium-119 ingredients, liquid inputs, stock strengths/doses and preparation scopes; preserve source-specific variants, quantity limits and disagreements, and repair reciprocal family references without inferring growth. |