mrs_medium

Overview

External ontology

komodo.medium:11 (source link unavailable) — MRS medium

Applications

• Microbial cultivation

pH range

{'min': 6.2, 'max': 6.5}

Notes

Source: KOMODO ModelSEED | ID: 11 | DSMZ Medium: 11 (mediadive.medium:11) | Aerobic: No

Composition

flowchart LR
    CultureMech_003940["mrs_medium"]
    ing0_Casein_peptone["Casein peptone"]
    CultureMech_003940 --> ing0_Casein_peptone
    ing1_Meat_extract["Meat extract"]
    CultureMech_003940 --> ing1_Meat_extract
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_003940 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_003940 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Tween_80["Tween 80"]
    CultureMech_003940 --> ing4_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing4_Tween_80 -.-> CHEBI_53426
    ing5_K2HPO4["K2HPO4"]
    CultureMech_003940 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Na_acetate["Na-acetate"]
    CultureMech_003940 --> ing6_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Na_acetate -.-> CHEBI_32954
    ing7__NH4_3_citrate["(NH4)3 citrate"]
    CultureMech_003940 --> ing7__NH4_3_citrate
    CHEBI_63037["CHEBI:63037"]
    ing7__NH4_3_citrate -.-> CHEBI_63037
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003940 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_003940 --> ing9_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing9_MnSO4_x_H2O -.-> CHEBI_86364
    ing10_Biotin["Biotin"]
    CultureMech_003940 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Folic_acid["Folic acid"]
    CultureMech_003940 --> ing11_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing11_Folic_acid -.-> CHEBI_27470
    ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003940 --> ing12_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_003940 --> ing13_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing14_Riboflavin["Riboflavin"]
    CultureMech_003940 --> ing14_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing14_Riboflavin -.-> CHEBI_17015
    ing15_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003940 --> ing15_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing15_Nicotinic_acid -.-> CHEBI_15940
    ing16_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003940 --> ing16_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing16_Calcium_pantothenate -.-> CHEBI_31345
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003940 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_003940 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843

Ingredients (19)

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Heyndrickxia coagulans

  Term

  Id

  NCBITaxon:1121088

  Label

  Heyndrickxia coagulans DSM 1 = ATCC 7050

  Genome assembly id

  • GCA_000832905.1
  • GCF_000832905.1

  Strain

  ATCC 7050; DSM 1

  Growth metrics

  • Doubling time minutes

    79.8

    Measurement conditions

    MRS medium; anaerobic conditions; source compared MRS with M17 and evaluated oxygen concentration effects.

    Evidence

    • Reference

      PMID:39353547

      Supports

      SUPPORT

      Snippet

      H. coagulans ATCC 7050 exhibited its minimum doubling time of 79.8 ± 0.64 min in anaerobic conditions

      Explanation

      Manual literature review found direct strain-specific doubling-time evidence for Heyndrickxia coagulans ATCC 7050 in the MRS selected-medium oxygen-condition experiment.

    Is max attainment

    False

• Preferred term

  Lactiplantibacillus plantarum BG24

  Term

  Id

  NCBITaxon:337330

  Label

  Lactiplantibacillus plantarum subsp. plantarum

  Strain

  BG24

  Growth metrics

  • Max od600

    6.6

    Measurement conditions

    MRS broth; static culture compared with 100 rpm and 200 rpm shaking.

    Evidence

    • Reference

      PMID:38194015

      Supports

      SUPPORT

      Snippet

      L. plantarum BG24 strain showed higher microbial growth under static conditions (6.60 OD600) compared to 100 rpm (5.73 OD600) and 200 rpm (5.02 OD600) shaking speed due to its facultative anaerobic characteristic.

      Explanation

      The source directly reports strain-specific growth of Lactiplantibacillus plantarum BG24 in MRS broth under static and shaken culture conditions.

    Is max attainment

    True

  • Growth rate per hour

    0.416

    Doubling time minutes

    100.2

    Ph at measurement

    5.7

    Measurement conditions

    Original MRS broth, pH 5.7; source also reports biomass productivity of 0.14 g/L/h.

    Evidence

    • Reference

      PMID:38194015

      Supports

      SUPPORT

      Snippet

      The specific growth rate of L. plantarum BG24 was 0.416 h-1, the doubling time was 1.67 h, and the biomass productivity value was 0.14 gL-1 h-1 in the original MRS broth (pH 5.7)

      Explanation

      The source directly reports growth rate and doubling time for Lactiplantibacillus plantarum BG24 in original MRS broth.

    Is max attainment

    False

  • Growth rate per hour

    0.483

    Doubling time minutes

    85.8

    Ph at measurement

    6.5

    Measurement conditions

    MRS broth at pH 6.5 enriched with 5 g/L yeast extract; source also reports biomass productivity of 0.17 g/L/h.

    Evidence

    • Reference

      PMID:38194015

      Supports

      SUPPORT

      Snippet

      higher values were found as 0.483 h-1, 1.43 h and 0.17 gL-1 h-1, respectively, in MRS broth (pH 6.5) medium enriched with 5 g/L yeast extract.

      Explanation

      The source directly reports improved growth rate and doubling time for Lactiplantibacillus plantarum BG24 in a pH-adjusted, yeast-extract-enriched MRS broth variant.

    Is max attainment

    True

  Evidence

  • Reference

    PMID:38194015

    Supports

    SUPPORT

    Explanation

    The abstract identifies Lactiplantibacillus plantarum BG24 and reports quantitative growth optimization in original and modified MRS broth.

• Preferred term

  Lactiplantibacillus plantarum

  Term

  Id

  NCBITaxon:337330

  Label

  Lactiplantibacillus plantarum subsp. plantarum

  Strain

  ATCC 10012; DSM 20246; NCDO 352; NCIB 8016

  Growth metrics

  • Doubling time minutes

    85.5

    Measurement conditions

    MRS medium; microaerophilic conditions; source compared MRS with M17 and evaluated oxygen concentration effects.

    Evidence

    • Reference

      PMID:39353547

      Supports

      SUPPORT

      Snippet

      L.plantarum ATCC 10012 demonstrated its minimum doubling time of 85.5 ± 1.2 min under microaerophilic conditions

      Explanation

      Manual literature review found direct strain-specific doubling-time evidence for Lactiplantibacillus plantarum ATCC 10012 in the MRS selected-medium oxygen-condition experiment.

    Is max attainment

    False

Recipe variants

• Name

  mrs_bsh_oxygen_condition_atcc7050_atcc10012

  Description

  MRS medium condition used to compare oxygen-level effects on bile salt hydrolase production and growth kinetics in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012.

  Modifications

  • Compare MRS medium with M17 medium for bile salt hydrolase production.
  • Evaluate oxygen concentration effects in MRS medium, including anaerobic and microaerophilic conditions.

  Purpose

  Capture strain-specific MRS growth and doubling-time evidence under the parent MRS medium record.

  Evidence

  • Reference

    PMID:39353547

    Supports

    SUPPORT

    Snippet

    Cultivation of cultures in MRS and M17 media revealed that MRS medium enhanced BSH production by 235.98 % in H. coagulans ATCC 7050 and 147.37 % in L. plantarum ATCC 10012, compared to M 17 medium.

    Explanation

    The source directly compares growth-associated fermentation performance of the two named ATCC strains in MRS versus M17 and then reports oxygen-condition doubling times for the same strains.

• Name

  mrs_broth_bg24_ph_yeast_extract_optimization

  Description

  MRS broth growth optimization condition for Lactiplantibacillus plantarum BG24, comparing static and shaken cultures and an MRS broth condition adjusted to pH 6.5 with additional yeast extract.

  Modifications

  • Compare static culture with 100 rpm and 200 rpm shaking in MRS broth.
  • Compare original MRS broth at pH 5.7 with MRS broth at pH 6.5 enriched with 5 g/L yeast extract.

  Purpose

  Capture strain-specific BG24 MRS broth growth optimization evidence under the shared MRS parent medium.

  Evidence

  • Reference

    PMID:38194015

    Supports

    SUPPORT

    Snippet

    The specific growth rate of L. plantarum BG24 was 0.416 h-1, the doubling time was 1.67 h, and the biomass productivity value was 0.14 gL-1 h-1 in the original MRS broth (pH 5.7) while higher values were found as 0.483 h-1, 1.43 h and 0.17 gL-1 h-1, respectively, in MRS broth (pH 6.5) medium enriched with 5 g/L yeast extract.

    Explanation

    The source directly reports quantitative growth kinetics for Lactiplantibacillus plantarum BG24 in original and modified MRS broth.

Provenance