oteb_medium (CultureMech:004003)

Overview

External ontology

komodo.medium:1245 (source link unavailable) — OTEB-MEDIUM

Applications

Microbial cultivation

pH range

{'min': 6.8, 'max': 7.2}

Notes

Source: KOMODO ModelSEED | ID: 1245 | DSMZ Medium: 1245 (mediadive.medium:1245) | Aerobic: No Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preserve the exact KOMODO Gram Amounts and nulls as source-specific assertions. No provider molar aggregation or copied DSMZ formulation replaces these rows. pH metadata is retained without inferring a missing value. Complete source tables were inspected. Reconstructed ingredient/stock nodes retain source-specific amounts and uncertainties; historical flattened amounts, false supplier-tagged LB ingredients and unsupported interpretations are archived in append-only history. Existing unrelated metadata and prior evidence remain preserved, not recertified.

Composition

flowchart LR
    CultureMech_004003["oteb_medium"]
    ing0_Isobutyric_Acid["Isobutyric Acid"]
    CultureMech_004003 --> ing0_Isobutyric_Acid
    CHEBI_16135["CHEBI:16135"]
    ing0_Isobutyric_Acid -.-> CHEBI_16135
    ing1_Distilled_Water["Distilled Water"]
    CultureMech_004003 --> ing1_Distilled_Water
    ing2_Sucrose["Sucrose"]
    CultureMech_004003 --> ing2_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing2_Sucrose -.-> CHEBI_17992
    ing3_Proteose_Peptone_no__2["Proteose Peptone no. 2"]
    CultureMech_004003 --> ing3_Proteose_Peptone_no__2
    ing4_Magnesium_Sulfate["Magnesium Sulfate"]
    CultureMech_004003 --> ing4_Magnesium_Sulfate
    CHEBI_32599["CHEBI:32599"]
    ing4_Magnesium_Sulfate -.-> CHEBI_32599
    ing5_Glucose["Glucose"]
    CultureMech_004003 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Yeast_Extract["Yeast Extract"]
    CultureMech_004003 --> ing6_Yeast_Extract
    ing7_Calf_Serum["Calf Serum"]
    CultureMech_004003 --> ing7_Calf_Serum
    ing8_Heart_Infusion_Broth["Heart Infusion Broth"]
    CultureMech_004003 --> ing8_Heart_Infusion_Broth
    ing9_Thiamine_Pyrophosphate["Thiamine Pyrophosphate"]
    CultureMech_004003 --> ing9_Thiamine_Pyrophosphate
    ing10_Acetic_Acid["Acetic Acid"]
    CultureMech_004003 --> ing10_Acetic_Acid
    CHEBI_15366["CHEBI:15366"]
    ing10_Acetic_Acid -.-> CHEBI_15366
    ing11_Ribose["Ribose"]
    CultureMech_004003 --> ing11_Ribose
    CHEBI_33942["CHEBI:33942"]
    ing11_Ribose -.-> CHEBI_33942
    ing12_L_Cysteine["L-Cysteine"]
    CultureMech_004003 --> ing12_L_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing12_L_Cysteine -.-> CHEBI_17561
    ing13_Propionic_Acid["Propionic Acid"]
    CultureMech_004003 --> ing13_Propionic_Acid
    CHEBI_30768["CHEBI:30768"]
    ing13_Propionic_Acid -.-> CHEBI_30768
    ing14_N_Butyric_Acid["N-Butyric Acid"]
    CultureMech_004003 --> ing14_N_Butyric_Acid
    CHEBI_30772["CHEBI:30772"]
    ing14_N_Butyric_Acid -.-> CHEBI_30772
    ing15_Sodium_Chloride["Sodium Chloride"]
    CultureMech_004003 --> ing15_Sodium_Chloride
    CHEBI_26710["CHEBI:26710"]
    ing15_Sodium_Chloride -.-> CHEBI_26710
    ing16_Starch["Starch"]
    CultureMech_004003 --> ing16_Starch
    CHEBI_28017["CHEBI:28017"]
    ing16_Starch -.-> CHEBI_28017
    ing17_N_Valeric_Acid["N-Valeric Acid"]
    CultureMech_004003 --> ing17_N_Valeric_Acid
    CHEBI_17418["CHEBI:17418"]
    ing17_N_Valeric_Acid -.-> CHEBI_17418
    ing18_Vitamin_K1["Vitamin K1"]
    CultureMech_004003 --> ing18_Vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing18_Vitamin_K1 -.-> CHEBI_18067
    ing19_Pectin["Pectin"]
    CultureMech_004003 --> ing19_Pectin
    CHEBI_17309["CHEBI:17309"]
    ing19_Pectin -.-> CHEBI_17309
    ing20_Maltose["Maltose"]
    CultureMech_004003 --> ing20_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing20_Maltose -.-> CHEBI_17306
    ing21_Fructose["Fructose"]
    CultureMech_004003 --> ing21_Fructose
    CHEBI_28757["CHEBI:28757"]
    ing21_Fructose -.-> CHEBI_28757
    ing22_Xylose["Xylose"]
    CultureMech_004003 --> ing22_Xylose
    CHEBI_18222["CHEBI:18222"]
    ing22_Xylose -.-> CHEBI_18222
    ing23_Isovaleric_Acid["Isovaleric Acid"]
    CultureMech_004003 --> ing23_Isovaleric_Acid
    CHEBI_28484["CHEBI:28484"]
    ing23_Isovaleric_Acid -.-> CHEBI_28484
    ing24_2_Methylbutyric_Acid["2-Methylbutyric Acid"]
    CultureMech_004003 --> ing24_2_Methylbutyric_Acid
    ing25_Potassium_Phosphate__dibasic["Potassium Phosphate, dibasic"]
    CultureMech_004003 --> ing25_Potassium_Phosphate__dibasic
    ing26_Sodium_Pyruvate["Sodium Pyruvate"]
    CultureMech_004003 --> ing26_Sodium_Pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing26_Sodium_Pyruvate -.-> CHEBI_50144

Ingredients (27)

#	Ingredient	Concentration	Ontology	Notes
1	Isobutyric Acid C4H8O2 88.106 g/mol		CHEBI:16135 isobutyric acid	The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
2	Distilled Water			The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
3	Sucrose C12H22O11 342.297 g/mol	0.80 G_PER_L	CHEBI:17992 sucrose	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
4	Proteose Peptone no. 2	4.98 G_PER_L		Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
5	Magnesium Sulfate Mg.O4S 120.368 g/mol	0.10 G_PER_L	CHEBI:32599 magnesium sulfate	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
6	Glucose C6H12O6 180.156 g/mol	0.80 G_PER_L	CHEBI:17234 glucose	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
7	Yeast Extract	4.98 G_PER_L		Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
8	Calf Serum	99.70 G_PER_L		Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium. Keep source serum labels distinct. The strain variant includes both calf serum 99.70 and an additional heat-inactivated fetal-bovine-serum row 100.00; neither substitution nor equivalence is established.
9	Heart Infusion Broth	4.98 G_PER_L		Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
10	Thiamine Pyrophosphate	7.48E-5 G_PER_L		Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium. The row's name says thiamine pyrophosphate but its semi-unique name says Thiamine-HCl. Retain that explicit provider identity conflict in notes; do not use the alias or a copied chloride annotation to establish chemical form or molecular weight.
11	Acetic Acid C2H4O2 60.052 g/mol		CHEBI:15366 acetic acid	The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
12	Ribose C5H10O5 150.13 g/mol	0.80 G_PER_L	CHEBI:33942 ribose	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
13	L-Cysteine C3H7NO2S 121.161 g/mol	0.50 G_PER_L	CHEBI:17561 L-cysteine	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
14	Propionic Acid C3H6O2 74.079 g/mol		CHEBI:30768 propionic acid	The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
15	N-Butyric Acid C4H8O2 88.106 g/mol		CHEBI:30772 butyric acid	The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
16	Sodium Chloride Cl.Na 58.443 g/mol	4.98 G_PER_L	CHEBI:26710 sodium chloride	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
17	Starch	0.80 G_PER_L	CHEBI:28017 starch	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
18	N-Valeric Acid C5H10O2 102.133 g/mol		CHEBI:17418 valeric acid	The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
19	Vitamin K1 C31H46O2 450.707 g/mol		CHEBI:18067 phylloquinone	The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
20	Pectin	0.80 G_PER_L	CHEBI:17309 pectin	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
21	Maltose C12H22O11 342.297 g/mol	1.60 G_PER_L	CHEBI:17306 maltose	Superseded flattening history, not a current amount: [Collapsed 2 identical duplicates: 8.0, 8.0] Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium. This displayed rate is source-specific. It does not decide whether the original's two separate 8 g rows are intentional or duplicated.
22	Fructose	0.80 G_PER_L	CHEBI:28757 fructose	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
23	Xylose C5H10O5 150.13 g/mol	0.80 G_PER_L	CHEBI:18222 xylose	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
24	Isovaleric Acid C5H10O2 102.133 g/mol		CHEBI:28484 isovaleric acid	The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
25	2-Methylbutyric Acid			The exact Gram Amount is null. Remove a copied numeric assertion if present; retain the named ingredient without a fixed concentration. Do not infer zero, density, or a quantity from the molar aggregation. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.
26	Potassium Phosphate, dibasic	1.99 G_PER_L		(dibasic) Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium. Dibasic phosphate is not the copied monobasic potassium dihydrogen phosphate annotation. Withdraw that inexact annotation without inventing a replacement identifier.
27	Sodium Pyruvate C3H3O3.Na 110.044 g/mol	0.80 G_PER_L	CHEBI:50144 sodium pyruvate	Keep the exact displayed Gram Amount and precision under the provider's per-liter heading. It is a provider assertion, not an independent final-volume measurement. Mol Amount and combined SEED rows are not substitutes for this field. Exact per-liter heading: Gram & Mol amounts are per Liter of medium.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:01.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 1245
2026-02-03T11:18:59.204026	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 1245
2026-03-13T03:08:49.825227Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:24.042755Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004003
2026-03-17T06:29:53.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:38.698456+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "OTEB-MEDIUM" to "oteb_medium" for consistent matching
2026-05-20T06:00:49.748820+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '6.8-7.2' -> {'min': 6.8, 'max': 7.2}
2026-06-05T07:40:20.369946+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-02T07:37:35.686508+00:00	repair_merged_duplicates.py	REPAIRED_SUMMED_DUPLICATE_MERGE	Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.
2026-10-09T06:18:47.388455+00:00	codex	RESTORED_OTEB_SOURCE_FORMULATIONS_AND_STOCK_SCOPES_WITH_EVIDENCE	Restore exact-source OTEB formulation and stock scopes, preserve provider rates/nulls and original source conflicts, correct input units and family links, and retain prior evidence/history. No biological growth association added.