Overview
- External ontology
- komodo.medium:1264.1 (source link unavailable) — for DSM 14994
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: KOMODO ModelSEED | ID: 1264.1 | DSMZ Medium: 1264 (mediadive.medium:1264) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004020["for_dsm_14994"]
ing0_NaCl["NaCl"]
CultureMech_004020 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NH4Cl["NH4Cl"]
CultureMech_004020 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004020 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgCl2_x_2_H2O["MgCl2 x 2 H2O"]
CultureMech_004020 --> ing3_MgCl2_x_2_H2O
CHEBI_131394["CHEBI:131394"]
ing3_MgCl2_x_2_H2O -.-> CHEBI_131394
ing4_Starch["Starch"]
CultureMech_004020 --> ing4_Starch
CHEBI_28017["CHEBI:28017"]
ing4_Starch -.-> CHEBI_28017
ing5_Yeast_extract["Yeast extract"]
CultureMech_004020 --> ing5_Yeast_extract
ing6_Peptone["Peptone"]
CultureMech_004020 --> ing6_Peptone
ing7_Phosphate_buffer["Phosphate buffer"]
CultureMech_004020 --> ing7_Phosphate_buffer
ing8_Resazurin["Resazurin"]
CultureMech_004020 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Na2S["Na2S"]
CultureMech_004020 --> ing9_Na2S
CHEBI_76208["CHEBI:76208"]
ing9_Na2S -.-> CHEBI_76208
ing10_Vitamine_B12["Vitamine B12"]
CultureMech_004020 --> ing10_Vitamine_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamine_B12 -.-> CHEBI_176843
ing11_EDTA["EDTA"]
CultureMech_004020 --> ing11_EDTA
CHEBI_4735["CHEBI:4735"]
ing11_EDTA -.-> CHEBI_4735
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004020 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004020 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_H3BO3["H3BO3"]
CultureMech_004020 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004020 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004020 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004020 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004020 --> ing18_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004020 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgCl2 x 2 H2O
2Cl.2H2O.Mg 131.241 g/mol
|
3 G_PER_L |
CHEBI:131394
magnesium dichloride dihydrate
|
|
| 5 | Starch | 5 G_PER_L |
CHEBI:28017
starch
|
|
| 6 | Yeast extract | 1 G_PER_L | ||
| 7 | Peptone | 5 G_PER_L | ||
| 8 | Phosphate buffer | 1 G_PER_L | ||
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2S
2Na.S 78.047 g/mol
|
0.003 G_PER_L |
CHEBI:76208
sodium sulfide (anhydrous)
|
|
| 11 | Vitamine B12 | 50 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 12 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.01 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.02 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.002 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.003 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.001 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1264.1 |
| 2026-02-03T11:18:56.863979 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1264 |
| 2026-03-13T06:19:24.196859Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004020 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:38.883118+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "for DSM 14994" to "for_dsm_14994" for consistent matching |
| 2026-06-05T07:41:01.911606+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:57.443909+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_1264_spirochaeta_score10.py | RESOLVED_KOMODO_1264_SPIROCHAETA_TOPOLOGY | KOMODO Medium 1264.1 applies DSMZ Medium 1264 to DSM 14994. |