Overview
- External ontology
- komodo.medium:1282 (source link unavailable) — SH SEAWATER medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.5, 'max': 8.5}
- Notes
- Source: KOMODO ModelSEED | ID: 1282 | DSMZ Medium: 1282 (mediadive.medium:1282) | Aerobic: No
Composition
flowchart LR
CultureMech_004034["sh_seawater_medium"]
ing0_Peptone["Peptone"]
CultureMech_004034 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_004034 --> ing1_Yeast_extract
ing2_NH4NO3["NH4NO3"]
CultureMech_004034 --> ing2_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing2_NH4NO3 -.-> CHEBI_63038
ing3_NaCl["NaCl"]
CultureMech_004034 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_004034 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004034 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_KCl["KCl"]
CultureMech_004034 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Na2SO4["Na2SO4"]
CultureMech_004034 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_HEPES["HEPES"]
CultureMech_004034 --> ing8_HEPES
CHEBI_46756["CHEBI:46756"]
ing8_HEPES -.-> CHEBI_46756
ing9_KBr["KBr"]
CultureMech_004034 --> ing9_KBr
CHEBI_32030["CHEBI:32030"]
ing9_KBr -.-> CHEBI_32030
ing10_H3BO3["H3BO3"]
CultureMech_004034 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_SrCl2["SrCl2"]
CultureMech_004034 --> ing11_SrCl2
CHEBI_36383["CHEBI:36383"]
ing11_SrCl2 -.-> CHEBI_36383
ing12_NH4Cl["NH4Cl"]
CultureMech_004034 --> ing12_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing12_NH4Cl -.-> CHEBI_31206
ing13_KH2PO4["KH2PO4"]
CultureMech_004034 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
ing14_NaF["NaF"]
CultureMech_004034 --> ing14_NaF
CHEBI_28741["CHEBI:28741"]
ing14_NaF -.-> CHEBI_28741
ing15_NaHCO3["NaHCO3"]
CultureMech_004034 --> ing15_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing15_NaHCO3 -.-> CHEBI_32139
ing16_Agar["Agar"]
CultureMech_004034 --> ing16_Agar
CHEBI_2509["CHEBI:2509"]
ing16_Agar -.-> CHEBI_2509
ing17_HCl["HCl"]
CultureMech_004034 --> ing17_HCl
CHEBI_17883["CHEBI:17883"]
ing17_HCl -.-> CHEBI_17883
ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_004034 --> ing18_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004034 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
CultureMech_004034 --> ing20_MnCl2_x_2_H2O
CHEBI_131395["CHEBI:131395"]
ing20_MnCl2_x_2_H2O -.-> CHEBI_131395
ing21_ZnCl2["ZnCl2"]
CultureMech_004034 --> ing21_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing21_ZnCl2 -.-> CHEBI_49976
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004034 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004034 --> ing23_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004034 --> ing24_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
ing25_Double_distilled_water["Double distilled water"]
CultureMech_004034 --> ing25_Double_distilled_water
CHEBI_15377["CHEBI:15377"]
ing25_Double_distilled_water -.-> CHEBI_15377
ing26_Para_aminobenzoic_acid["Para-aminobenzoic acid"]
CultureMech_004034 --> ing26_Para_aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_Para_aminobenzoic_acid -.-> CHEBI_30753
ing27_Folic_acid["Folic acid"]
CultureMech_004034 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Biotin["Biotin"]
CultureMech_004034 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_004034 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_004034 --> more["...6 more"]
Ingredients (36)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.2 G_PER_L | ||
| 2 | Yeast extract | 0.1 G_PER_L | ||
| 3 |
NH4NO3
H4N.NO3 80.043 g/mol
|
0.0032 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
24.32 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.66 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4.0 G_PER_L |
CHEBI:32149
sodium sulfate
|
[Collapsed 2 identical duplicates: 4.0, 4.0] |
| 9 | HEPES | 2.38 G_PER_L |
CHEBI:46756
HEPES
|
|
| 10 |
KBr
Br.K 119.002 g/mol
|
0.09996 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.025336 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.024736, 0.0006] |
| 12 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.023778 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 13 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.021396 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0054434 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 15 |
NaF
F.Na 41.988 g/mol
|
0.00293917 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 16 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 17 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 18 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 19 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
|
0.1 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
|
| 22 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.036 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
10 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 25 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 26 |
Double distilled water
H2O 18.015 g/mol
|
1990.0 G_PER_L |
CHEBI:15377
water
|
[Merged 2 duplicates: 990.0, 1000.0] |
| 27 |
Para-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.025 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.01 G_PER_L |
CHEBI:27470
folic acid
|
|
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.025 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.025 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 33 |
Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.025 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 34 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.025 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 35 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
0.025 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
|
| 36 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.025 G_PER_L |
CHEBI:16494
lipoic acid
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1282 |
| 2026-02-03T11:19:14.113497 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1282 |
| 2026-03-13T03:09:30.039484Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:24.330533Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004034 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.051838+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SH SEAWATER medium" to "sh_seawater_medium" for consistent matching |
| 2026-05-20T06:00:49.928885+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.5-8.5' -> {'min': 7.5, 'max': 8.5} |
| 2026-06-05T07:40:20.627222+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.033322+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:35.850965+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |