Overview
- External ontology
- komodo.medium:1291 (source link unavailable) — T49 medium
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: KOMODO ModelSEED | ID: 1291 | DSMZ Medium: 1291 (mediadive.medium:1291) | Aerobic: No
Composition
flowchart LR
CultureMech_004041["t49_medium"]
ing0_Gellan_Gum["Gellan Gum"]
CultureMech_004041 --> ing0_Gellan_Gum
CHEBI_85248["CHEBI:85248"]
ing0_Gellan_Gum -.-> CHEBI_85248
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_004041 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_K2HPO4["K2HPO4"]
CultureMech_004041 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004041 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_004041 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Yeast_extract["Yeast extract"]
CultureMech_004041 --> ing5_Yeast_extract
ing6_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_004041 --> ing6_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing6_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing7_ZnCl2["ZnCl2"]
CultureMech_004041 --> ing7_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing7_ZnCl2 -.-> CHEBI_49976
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004041 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_004041 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004041 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004041 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004041 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004041 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_004041 --> ing14_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
ing15_D_____biotin["D-(+)-biotin"]
CultureMech_004041 --> ing15_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing15_D_____biotin -.-> CHEBI_15956
ing16_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_004041 --> ing16_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Gellan Gum | 0.5 G_PER_L |
CHEBI:85248
gellan gum
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
4 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.45 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 | Yeast extract | 0.01 G_PER_L | ||
| 7 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 8 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 16 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 17 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Solutions
Trace element solution SL-11
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 1.5 G_PER_L |
Seven vitamins solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 0.1 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | |
| Pyridoxine hydrochloride | 0.3 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1291 |
| 2026-02-03T11:19:11.298215 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1291 |
| 2026-03-13T06:19:24.377914Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004041 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.106553+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "T49 medium" to "t49_medium" for consistent matching |
| 2026-06-05T07:41:48.662110+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.463860+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:31.957980+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution SL-11' @ 1 ml/l; 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-08-15T05:35:03.783751+00:00 | promote_komodo_volume_candidates.py | ASSERTED_VOLUME_FROM_COMPOSITION_MATCH | This record reproduces MediaDive medium 1291 ('CHTHONOMONAS MEDIUM') across 21 shared compounds at 100% identical values. That identifies the medium despite the name difference, so its stated addition volume is now asserted rather than proposed (#262). |