Overview
- External ontology
- komodo.medium:12 (source link unavailable) — SOIL EXTRACT medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.8, 'max': 7.0}
- Notes
- Source: KOMODO ModelSEED | ID: 12 | DSMZ Medium: 12 (mediadive.medium:12) | Aerobic: No
Composition
flowchart LR
CultureMech_004048["soil_extract_medium"]
ing0_Agar["Agar"]
CultureMech_004048 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
15 g per liter of clarified supernatant, not per liter of the starting soil suspension. |
Preparation
- Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained.
- Adjust pH to 6.8 - 7.0 and sterilize.
Solutions
Clarified soil extract
| Ingredient | Concentration | Notes |
|---|---|---|
| air-dried garden soil | Extraction starting material: 400 g in 1000 ml tap water. Solids are separated before the recovered extract is used. The source does not quantify retained solids or dissolved yield; no numeric extract concentration is asserted. | |
| Tap water | 1 L | Initial extraction-water addition: 1000 ml / 1000 ml per L = 1 L, not a mass concentration or a measured recovered volume. |
Curator-described extraction stage, not a separately accessioned stock. Composition lists preparation inputs, not retained solutes. Recovered volume and an absolute extract dose are not specified; agar is dosed per liter of clarified supernatant.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 12 |
| 2026-02-03T11:19:16.123840 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 12 |
| 2026-03-13T06:19:24.441282Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004048 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.182028+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SOIL EXTRACT medium" to "soil_extract_medium" for consistent matching |
| 2026-05-20T06:00:49.995914+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6.8-7.0' -> {'min': 6.8, 'max': 7.0} |
| 2026-06-05T07:40:20.706898+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T13:10:26.050604+00:00 | codex | CORRECTED_KOMODO12_EXTRACTION_FROM_VERIFIED_SOURCE_LINK | Original KOMODO source link and DSMZ recipe verified. Removed unsupported soil concentration, retained input mass in notes, corrected initial water to 1 L, and restored extraction/agar preparation. No recovered volume, extract yield, or absolute extract dose inferred. |