Overview
- External ontology
- komodo.medium:1313 (source link unavailable) — MARINE AMMONIUM MINERAL SALTS
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 1313 | DSMZ Medium: 1313 (mediadive.medium:1313) | Aerobic: No
Composition
flowchart LR
CultureMech_004069["marine_ammonium_mineral_salts"]
ing0_NaCl["NaCl"]
CultureMech_004069 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_004069 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004069 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004069 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004069 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_004069 --> ing5_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing5_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004069 --> ing6_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing7_KH2PO4["KH2PO4"]
CultureMech_004069 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_K2HPO4["K2HPO4"]
CultureMech_004069 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_HCl["HCl"]
CultureMech_004069 --> ing9_HCl
CHEBI_17883["CHEBI:17883"]
ing9_HCl -.-> CHEBI_17883
ing10_ZnCl2["ZnCl2"]
CultureMech_004069 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004069 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_004069 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004069 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004069 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004069 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Para_aminobenzoic_acid["Para-aminobenzoic acid"]
CultureMech_004069 --> ing16_Para_aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_Para_aminobenzoic_acid -.-> CHEBI_30753
ing17_Biotin["Biotin"]
CultureMech_004069 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_004069 --> ing18_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_pantothenate -.-> CHEBI_31345
ing19_Lipoic_acid["Lipoic acid"]
CultureMech_004069 --> ing19_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19_Lipoic_acid -.-> CHEBI_16494
ing20_Folic_acid["Folic acid"]
CultureMech_004069 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_NaOH["NaOH"]
CultureMech_004069 --> ing21_NaOH
CHEBI_32145["CHEBI:32145"]
ing21_NaOH -.-> CHEBI_32145
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
22.2222 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.11111 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.222222 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.11111 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00222222 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.00333333 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 7 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0580431 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Merged 2 duplicates: 0.0222222, 0.0358209] |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
23.4 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
HCl
HCl 36.461 g/mol
|
5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0696517 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.199005 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.00597015 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.189055 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.00199005 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0238806 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Para-aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.3 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 20 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.05 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Solutions
Vitamin Solution V10
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nicotinic acid | 0.2 G_PER_L | |
| Pyridoxine hydrochloride | 0.5 G_PER_L | |
| Cyanocobalamin | 0.1 G_PER_L | |
| Thiamine | 0.2 G_PER_L | |
| Riboflavin | 0.1 G_PER_L |
Trace element solution SL10
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 1.49254 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1313 |
| 2026-02-03T11:19:09.007644 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1313 |
| 2026-03-13T03:09:15.792697Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s); Added pH buffer: NaOH |
| 2026-03-13T06:19:24.570027Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004069 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.333227+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MARINE AMMONIUM MINERAL SALTS" to "marine_ammonium_mineral_salts" for consistent matching |
| 2026-05-16T09:03:40.184705+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:12.822198+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.864077+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T20:44:33.873005+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 6 stock-strength component(s) out of ingredients into 2 solution(s): 5 -> 'Vitamin Solution V10' @ 1 ml/l; 1 -> 'Trace element solution SL10' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |