methanobacterium_thermoautotrophicum_medium (CultureMech:004074)

Overview

External ontology

komodo.medium:131 (source link unavailable) — METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM

Applications

Microbial cultivation

pH

7.2

Notes

Archived DSMZ Medium 131 defines METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM with base mineral salts, 10 mL/L vitamin solution, 10 mL/L trace element solution, resazurin, cysteine hydrochloride hydrate, sodium sulfide nonahydrate, pH 7.2, an 80:20 H2/CO2 anaerobic atmosphere, and N2-sterilized reducing solutions; this record expands those stock solutions into final per-liter components.

Composition

flowchart LR
    CultureMech_004074["methanobacterium_thermoautotrophicum_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_004074 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_004074 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_NaCl["NaCl"]
    CultureMech_004074 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004074 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004074 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_004074 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_K2HPO4["K2HPO4"]
    CultureMech_004074 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Na2CO3["Na2CO3"]
    CultureMech_004074 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_Na2_EDTA["Na2-EDTA"]
    CultureMech_004074 --> ing8_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing8_Na2_EDTA -.-> CHEBI_64734
    ing9_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_004074 --> ing9_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing9_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004074 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_004074 --> ing11_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing12_CuSO4["CuSO4"]
    CultureMech_004074 --> ing12_CuSO4
    CHEBI_23414["CHEBI:23414"]
    ing12_CuSO4 -.-> CHEBI_23414
    ing13_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
    CultureMech_004074 --> ing13_KAl_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing13_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing14_H3BO3["H3BO3"]
    CultureMech_004074 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004074 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004074 --> ing16_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing17_Biotin["Biotin"]
    CultureMech_004074 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_004074 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_004074 --> ing19_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_HCl -.-> CHEBI_30961
    ing20_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_004074 --> ing20_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing20_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing21_Riboflavine["Riboflavine"]
    CultureMech_004074 --> ing21_Riboflavine
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavine -.-> CHEBI_17015
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_004074 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Ca_pantothenate["Ca-pantothenate"]
    CultureMech_004074 --> ing23_Ca_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Ca_pantothenate -.-> CHEBI_31345
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_004074 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25_Vitamin_B12["Vitamin B12"]
    CultureMech_004074 --> ing25_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing25_Vitamin_B12 -.-> CHEBI_176843
    ing26_Resazurin["Resazurin"]
    CultureMech_004074 --> ing26_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing26_Resazurin -.-> CHEBI_8806
    ing27_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
    CultureMech_004074 --> ing27_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing27_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing28_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004074 --> ing28_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing28_Na2S_x_9_H2O -.-> CHEBI_76209
    ing29_H2["H2"]
    CultureMech_004074 --> ing29_H2
    CHEBI_18276["CHEBI:18276"]
    ing29_H2 -.-> CHEBI_18276
    CultureMech_004074 --> more["...3 more"]

Ingredients (33)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.300000 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 131 lists 0.300 g/L KH2PO4 per liter in the main recipe.
2	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.500000 G_PER_L	CHEBI:62946 ammonium sulfate	Archived DSMZ Medium 131 lists 1.500 g/L (NH4)2SO4 per liter in the main recipe.
3	NaCl Cl.Na 58.443 g/mol	0.610000 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 131 lists 0.600 g/L NaCl per liter in the main recipe and adds 10 mL/L of a trace element solution containing 1.000 g/L NaCl, yielding 0.610000 g/L total.
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.182000 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 131 lists 0.120 g/L MgSO4 x 7 H2O per liter in the main recipe and adds 10 mL/L of a trace element solution containing 6.200 g/L MgSO4 x 7 H2O, yielding 0.182000 g/L total.
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.081300 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 131 lists 0.080 g/L CaCl2 x 2 H2O per liter in the main recipe and adds 10 mL/L of a trace element solution containing 0.130 g/L CaCl2 x 2 H2O, yielding 0.081300 g/L total.
6	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.005000 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 131 lists 4.000 mg/L FeSO4 x 7 H2O per liter in the main recipe and adds 10 mL/L of a trace element solution containing 0.100 g/L FeSO4 x 7 H2O, yielding 0.005000 g/L total.
7	K2HPO4 HO4P.2K 174.174 g/mol	0.150000 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Archived DSMZ Medium 131 lists 0.150 g/L K2HPO4 per liter in the main recipe.
8	Na2CO3 CO3.2Na 105.988 g/mol	4.000000 G_PER_L	CHEBI:29377 sodium carbonate	Archived DSMZ Medium 131 lists 4.000 g/L Na2CO3 per liter in the main recipe.
9	Na2-EDTA C10H14N2O8.2Na 336.208 g/mol	0.006400 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.640 g/L Na2-EDTA, yielding 0.006400 g/L.
10	MnSO4 x 4 H2O 4H2O.Mn.O4S 223.061 g/mol	0.005500 G_PER_L	CHEBI:86358 manganese(II) sulfate tetrahydrate	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.550 g/L MnSO4 x 4 H2O, yielding 0.005500 g/L.
11	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.001700 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.170 g/L CoCl2 x 6 H2O, yielding 0.001700 g/L.
12	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.001800 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.180 g/L ZnSO4 x 7 H2O, yielding 0.001800 g/L.
13	CuSO4 Cu.O4S 159.609 g/mol	0.000500 G_PER_L	CHEBI:23414 copper(II) sulfate	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.050 g/L CuSO4, yielding 0.000500 g/L.
14	KAl(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.000180 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.018 g/L KAl(SO4)2 x 12 H2O, yielding 0.000180 g/L.
15	H3BO3 H3BO3 61.833 g/mol	0.000100 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.010 g/L H3BO3, yielding 0.000100 g/L.
16	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000110 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.011 g/L Na2MoO4 x 2 H2O, yielding 0.000110 g/L.
17	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.000250 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 131 adds 10 mL/L of a trace element solution containing 0.025 g/L NiCl2 x 6 H2O, yielding 0.000250 g/L.
18	Biotin C10H16N2O3S 244.316 g/mol	0.000020 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 2.000 mg/L Biotin, yielding 0.000020 g/L.
19	Folic acid C19H19N7O6 441.404 g/mol	0.000020 G_PER_L	CHEBI:27470 folic acid	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 2.000 mg/L Folic acid, yielding 0.000020 g/L.
20	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.000100 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 10.000 mg/L Pyridoxine-HCl, yielding 0.000100 g/L.
21	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000050 G_PER_L	CHEBI:132751 thiamine hydrochloride dihydrate	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 5.000 mg/L Thiamine-HCl x 2 H2O, yielding 0.000050 g/L.
22	Riboflavine C17H20N4O6 376.369 g/mol	0.000050 G_PER_L	CHEBI:17015 riboflavin	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 5.000 mg/L Riboflavine, yielding 0.000050 g/L.
23	Nicotinic acid C6H5NO2 123.111 g/mol	0.000050 G_PER_L	CHEBI:15940 nicotinic acid	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 5.000 mg/L Nicotinic acid, yielding 0.000050 g/L.
24	Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.000050 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 5.000 mg/L Ca-pantothenate, yielding 0.000050 g/L.
25	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000010 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 1.000 mg/L p-Aminobenzoic acid, yielding 0.000010 g/L.
26	Vitamin B12	0.000000100 G_PER_L	CHEBI:176843 vitamin B12	Archived DSMZ Medium 131 adds 10 mL/L of a vitamin solution containing 0.010 mg/L Vitamin B12, yielding 0.000000100 g/L.
27	Resazurin C12H7NO4 229.191 g/mol	0.001000 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 131 lists 1.000 mg/L Resazurin per liter in the main recipe.
28	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	1.500000 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Archived DSMZ Medium 131 lists 1.500 g/L Cysteine-HCl x H2O per liter in the main recipe.
29	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	1.500000 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 131 lists 1.500 g/L Na2S x 9 H2O per liter in the main recipe.
30	H2 H2 2.016 g/mol	variable VARIABLE	CHEBI:18276 dihydrogen	Archived DSMZ Medium 131 prepares and sterilizes the medium under 80% H2 and 20% CO2.
31	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 131 prepares and sterilizes the medium under 80% H2 and 20% CO2.
32	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 131 sterilizes the cysteine and sulfide solutions under N2.
33	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 131 lists 1000 mL distilled water in the main recipe.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 131
2026-03-13T06:19:24.621302Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004074
2026-03-17T19:45:39.395019+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM" to "methanobacterium_thermoautotrophicum_medium" for consistent matching
2026-08-06T08:58:32.351475+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_131_score30.py	RESOLVED_KOMODO_131_SCORE30	Archived DSMZ Medium 131 defines METHANOBACTERIUM THERMOAUTOTROPHICUM MEDIUM with base mineral salts, 10 mL/L vitamin solution, 10 mL/L trace element solution, resazurin, cysteine hydrochloride hydrate, sodium sulfide nonahydrate, pH 7.2, an 80:20 H2/CO2 anaerobic atmosphere, and N2-sterilized reducing solutions; this record expands those stock solutions into final per-liter components.
2026-09-13T00:00:00-07:00	repair_komodo_strain_backlinks.py	ADDED_KOMODO_STRAIN_BACKLINKS	Mirrored curated KOMODO parent_media links on the parent record.