Overview
- External ontology
- komodo.medium:1329 (source link unavailable) — METHANOSAETA PELAGICA medium
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: KOMODO ModelSEED | ID: 1329 | DSMZ Medium: 1329 (mediadive.medium:1329) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004085["methanosaeta_pelagica_medium"]
ing0_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_004085 --> ing0_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing0_MgCl2_x_6_H2O -.-> CHEBI_86345
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004085 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_NH4Cl["NH4Cl"]
CultureMech_004085 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_004085 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Na_acetate["Na-acetate"]
CultureMech_004085 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_Yeast_extract["Yeast extract"]
CultureMech_004085 --> ing5_Yeast_extract
ing6_Trypticase_peptone["Trypticase peptone"]
CultureMech_004085 --> ing6_Trypticase_peptone
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_004085 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_KH2PO4["KH2PO4"]
CultureMech_004085 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_K2HPO4["K2HPO4"]
CultureMech_004085 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_Na2CO3["Na2CO3"]
CultureMech_004085 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
CultureMech_004085 --> ing11_2_Mercaptoethanesulfonate
CHEBI_17905["CHEBI:17905"]
ing11_2_Mercaptoethanesulfonate -.-> CHEBI_17905
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004085 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_004085 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_004085 --> ing14_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004085 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004085 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_ZnCl2["ZnCl2"]
CultureMech_004085 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_CuCl2["CuCl2"]
CultureMech_004085 --> ing18_CuCl2
CHEBI_49553["CHEBI:49553"]
ing18_CuCl2 -.-> CHEBI_49553
ing19_H3BO3["H3BO3"]
CultureMech_004085 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004085 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004085 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_004085 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_004085 --> ing23_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing24_Biotin["Biotin"]
CultureMech_004085 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_004085 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_004085 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl["Thiamine HCl"]
CultureMech_004085 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Riboflavin["Riboflavin"]
CultureMech_004085 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_004085 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_004085 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.04088 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.249551 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.149551, 0.1] |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.538385 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
20.9402 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 19.9402, 1.0] |
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
6.54038 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 | Yeast extract | 1.49551 G_PER_L | ||
| 7 | Trypticase peptone | 0.398804 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498504 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.18644 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.209372 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.49551 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
2-Mercaptoethanesulfonate
C2H6O3S2 142.201 g/mol
|
0.139581 G_PER_L |
CHEBI:17905
coenzyme M
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.358923 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.03 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 19 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.02 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 24 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.04 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 34 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1329 |
| 2026-02-03T11:18:55.270851 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1329 |
| 2026-02-06T05:10:13.718542Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:40.336140Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:24.698192Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004085 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.484740+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOSAETA PELAGICA medium" to "methanosaeta_pelagica_medium" for consistent matching |
| 2026-06-05T07:40:20.917135+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.132411+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |