Overview
- External ontology
- komodo.medium:133.1 (source link unavailable) — For chemoautotrophic growth
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: KOMODO ModelSEED | ID: 133.1 | DSMZ Medium: 133 (mediadive.medium:133) | Aerobic: No
Composition
flowchart LR
CultureMech_004086["for_chemoautotrophic_growth"]
ing0_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_004086 --> ing0_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing0_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing1_KH2PO4["KH2PO4"]
CultureMech_004086 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_004086 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004086 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004086 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_004086 --> ing5_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing5_Ferric_ammonium_citrate -.-> CHEBI_31604
ing6_Na_acetate["Na-acetate"]
CultureMech_004086 --> ing6_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Na_acetate -.-> CHEBI_32954
ing7_Agar["Agar"]
CultureMech_004086 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004086 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004086 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_H3BO3["H3BO3"]
CultureMech_004086 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004086 --> ing11_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004086 --> ing12_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004086 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004086 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
4.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.75 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.03 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Ferric ammonium citrate | 0.018 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 7 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
3 G_PER_L |
CHEBI:32954
sodium acetate
|
Optional ingredient |
| 8 | Agar | 12 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 12 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 13 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 133.1 |
| 2026-02-03T11:19:09.589550 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 133 |
| 2026-03-13T06:19:24.708053Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004086 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.496367+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For chemoautotrophic growth" to "for_chemoautotrophic_growth" for consistent matching |
| 2026-06-05T07:41:00.550441+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.926344+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_133_carbon_monoxide_topology_score10.py | RESOLVED_KOMODO_133_CARBON_MONOXIDE_TOPOLOGY | KOMODO Medium 133.1 exactly matches the KOMODO Medium 133 MEDIUM FOR CARBON MONOXIDE OXIDIZERS base. |