Overview
- External ontology
- komodo.medium:1396_replace_Mineral salts solution for solid medium (medium 1396)_with_Mineral salts solution for liquid medium (medium 1396) (source link unavailable) — HALOARCHAEAL medium MH-1_replace_Mineral salts solution for solid medium (medium 1396)_with_Mineral salts solution for liquid medium (medium 1396)
- Applications
-
- Microbial cultivation
- pH
- 4.5
- High-metal flag
- True
- Notes
- Source: KOMODO ModelSEED | ID: 1396_replace_Mineral salts solution for solid medium (medium 1396)_with_Mineral salts solution for liquid medium (medium 1396) | DSMZ Medium: 1396 (mediadive.medium:1396) | Aerobic: No
Composition
flowchart LR
CultureMech_004113["haloarchaeal_medium_mh_1_replace_mineral_salts_solution_for_solid_m..."]
ing0_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_004113 --> ing0_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamic_acid -.-> CHEBI_16015
ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_004113 --> ing1_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing2_KH2PO4["KH2PO4"]
CultureMech_004113 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_004113 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_K2SO4["K2SO4"]
CultureMech_004113 --> ing4_K2SO4
CHEBI_32036["CHEBI:32036"]
ing4_K2SO4 -.-> CHEBI_32036
ing5_NaCl["NaCl"]
CultureMech_004113 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Casamino_acids["Casamino acids"]
CultureMech_004113 --> ing6_Casamino_acids
ing7_Yeast_extract["Yeast extract"]
CultureMech_004113 --> ing7_Yeast_extract
ing8_FeSO4_x_6_H2O["FeSO4 x 6 H2O"]
CultureMech_004113 --> ing8_FeSO4_x_6_H2O
CHEBI_75832["CHEBI:75832"]
ing8_FeSO4_x_6_H2O -.-> CHEBI_75832
ing9_Agar["Agar"]
CultureMech_004113 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004113 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004113 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_ZnCl2["ZnCl2"]
CultureMech_004113 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_H3BO4["H3BO4"]
CultureMech_004113 --> ing13_H3BO4
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004113 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004113 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_CuCl2_x_6_H2O["CuCl2 x 6 H2O"]
CultureMech_004113 --> ing16_CuCl2_x_6_H2O
CHEBI_49553["CHEBI:49553"]
ing16_CuCl2_x_6_H2O -.-> CHEBI_49553
ing17_AlCl3["AlCl3"]
CultureMech_004113 --> ing17_AlCl3
CHEBI_30114["CHEBI:30114"]
ing17_AlCl3 -.-> CHEBI_30114
ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_004113 --> ing18_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing19_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004113 --> ing19_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing19_CaCl2_x_2_H2O -.-> CHEBI_86158
ing20_BaCl2_x_2_H2O["BaCl2 x 2 H2O"]
CultureMech_004113 --> ing20_BaCl2_x_2_H2O
CHEBI_86153["CHEBI:86153"]
ing20_BaCl2_x_2_H2O -.-> CHEBI_86153
ing21_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_004113 --> ing21_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing21_Na2S2O3_x_5_H2O -.-> CHEBI_32150
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
2.49377 G_PER_L |
CHEBI:16015
L-glutamic acid
|
|
| 2 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2.49377 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.24688 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.24688 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
K2SO4
2K.O4S 174.259 g/mol
|
6.23441 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
249.377 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 | Casamino acids | 4.98753 G_PER_L | ||
| 8 | Yeast extract | 2.49377 G_PER_L | ||
| 9 |
FeSO4 x 6 H2O
Fe.O4S 151.908 g/mol
|
0.00498753 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 10 | Agar | 24.9377 G_PER_L |
CHEBI:2509
agar
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.3 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 | H3BO4 | 0.02 G_PER_L | ||
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
CuCl2 x 6 H2O
Cl2Cu 134.452 g/mol
|
0.01 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 18 |
AlCl3
AlCl3 133.341 g/mol
|
0.04 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 19 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.02 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 20 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 21 |
BaCl2 x 2 H2O
Ba.2Cl.2H2O 244.264 g/mol
|
0.01 G_PER_L |
CHEBI:86153
barium chloride dihydrate
|
|
| 22 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1396_replace_Mineral salts solution for solid medium (medium 1396)_with_Mineral salts solution for liquid medium (medium 1396) |
| 2026-02-03T11:19:13.602810 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1396 |
| 2026-03-13T06:19:24.949518Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004113 |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:39.781396+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOARCHAEAL medium MH-1_replace_Mineral salts solution for solid medium (medium 1396)_with_Mineral salts solution for liquid medium (medium 1396)" to "haloarchaeal_medium_mh_1_replace_mineral_salts_solution_for_solid_medium_medium_1396_with_mineral_salts_solution_for_liquid_medium_medium_1396" for consistent matching |
| 2026-06-05T07:41:07.486148+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.596888+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:59.437538+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |