methanogenium_medium

Overview

External ontology

komodo.medium:141 (source link unavailable) — METHANOGENIUM medium

Applications

• Microbial cultivation

pH range

{'min': 6.8, 'max': 7.0}

Notes

Source: KOMODO ModelSEED | ID: 141 | DSMZ Medium: 141 (mediadive.medium:141) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_004144["methanogenium_medium"]
    ing0_KCl["KCl"]
    CultureMech_004144 --> ing0_KCl
    CHEBI_32588["CHEBI:32588"]
    ing0_KCl -.-> CHEBI_32588
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_004144 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004144 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4Cl["NH4Cl"]
    CultureMech_004144 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004144 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_K2HPO4["K2HPO4"]
    CultureMech_004144 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_NaCl["NaCl"]
    CultureMech_004144 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_004144 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing8_Na_acetate["Na-acetate"]
    CultureMech_004144 --> ing8_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Na_acetate -.-> CHEBI_32954
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_004144 --> ing9_Yeast_extract
    ing10_Trypticase_peptone["Trypticase peptone"]
    CultureMech_004144 --> ing10_Trypticase_peptone
    ing11_Sodium_resazurin["Sodium resazurin"]
    CultureMech_004144 --> ing11_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Sodium_resazurin -.-> CHEBI_8806
    ing12_NaHCO3["NaHCO3"]
    CultureMech_004144 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_004144 --> ing13_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004144 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_004144 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_004144 --> ing16_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing16_MnSO4_x_H2O -.-> CHEBI_86364
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_004144 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_004144 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_004144 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_004144 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_004144 --> ing21_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing21_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing22_H3BO3["H3BO3"]
    CultureMech_004144 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004144 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004144 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_004144 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_004144 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing27_Biotin["Biotin"]
    CultureMech_004144 --> ing27_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_Biotin -.-> CHEBI_15956
    ing28_Folic_acid["Folic acid"]
    CultureMech_004144 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470
    ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_004144 --> ing29_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
    CultureMech_004144 --> more["...7 more"]

Ingredients (37)

Preparation

1. DSMZ Medium 141 notes that, when the medium is used without overpressure, pH should be adjusted with a small amount of sterile anoxic 1 N HCl if needed.
2. Dissolve ingredients except bicarbonate, vitamins, cysteine, and sulfide; sparge with 80% H2/20% CO2 for 30-45 min, add bicarbonate and adjust pH to 6.5, dispense under 80% H2/20% CO2 into anoxic Hungate tubes or serum vials to 30% of volume, and autoclave.
3. After sterilization, add cysteine and sulfide from sterile anoxic stocks autoclaved under 100% N2. Prepare vitamins under 100% N2 and filter-sterilize them before addition.
4. Adjust the complete medium to pH 6.8-7.0 if necessary, and incubate under sterile 80% H2/20% CO2 at two atmospheres of pressure.
5. For the Modified Wolin's mineral solution, first dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, add minerals, and adjust final pH to 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance