dilute_filtered_malt_extract_agar

CultureMech:004153 bacterial COMPLEX SOLID_AGAR
Original name: DILUTE FILTERED MALT EXTRACT AGAR

Overview

External ontology
komodo.medium:1427 (source link unavailable) — DILUTE FILTERED MALT EXTRACT AGAR
Applications
  • Microbial cultivation
Notes
DSMZ Medium 1427 prepares a 5.0 g/L malt extract filtrate, fills to 1.0 L, and adds 15.0 g agar.

Composition

flowchart LR
    CultureMech_004153["dilute_filtered_malt_extract_agar"]
    ing0_Malt_extract["Malt extract"]
    CultureMech_004153 --> ing0_Malt_extract
    ing1_Agar["Agar"]
    CultureMech_004153 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509
    ing2_Distilled_water["Distilled water"]
    CultureMech_004153 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Malt extract 5.0 G_PER_L DSMZ Medium 1427 extracts 5 g malt extract in 100 ml water and fills the filtered liquid to 1 L.
2 Agar 15.0 G_PER_L CHEBI:2509
agar
DSMZ Medium 1427 lists 15.0 g agar.
3 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
DSMZ Medium 1427 lists 1000 ml distilled water.

Preparation

  1. Mix 5 g malt extract with 100 ml distilled water.
  2. Autoclave for 15 min and cool.
  3. Filter until clear and fill to 1 L.
  4. Add 15 g agar.
  5. Autoclave at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 1427
2026-02-03T11:19:16.926589 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 1427
2026-03-13T06:19:25.446951Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004153
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:40.352512+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DILUTE FILTERED MALT EXTRACT AGAR" to "dilute_filtered_malt_extract_agar" for consistent matching
2026-06-05T07:40:58.718003+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_official_simple_score20.py RESOLVED_OFFICIAL_SIMPLE_SCORE20 DSMZ Medium 1427 prepares a 5.0 g/L malt extract filtrate, fills to 1.0 L, and adds 15.0 g agar.