Overview
- External ontology
- komodo.medium:153 (source link unavailable) — FLUID THIOGLYCOLATE medium
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- pH buffer: Na2CO3 | Source: KOMODO ModelSEED | ID: 153 | DSMZ Medium: 153 (mediadive.medium:153) | Aerobic: No
Composition
flowchart LR
CultureMech_004176["fluid_thioglycolate_medium"]
ing0_Trypticase["Trypticase"]
CultureMech_004176 --> ing0_Trypticase
ing1_L_Cystine["L-Cystine"]
CultureMech_004176 --> ing1_L_Cystine
CHEBI_16283["CHEBI:16283"]
ing1_L_Cystine -.-> CHEBI_16283
ing2_Glucose["Glucose"]
CultureMech_004176 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Yeast_extract["Yeast extract"]
CultureMech_004176 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_004176 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na_thioglycolate["Na-thioglycolate"]
CultureMech_004176 --> ing5_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing5_Na_thioglycolate -.-> CHEBI_86481
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_004176 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_Agar["Agar"]
CultureMech_004176 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Na2CO3["Na2CO3"]
CultureMech_004176 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase | 15 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
|
| 2 |
L-Cystine
C6H12N2O4S2 240.306 g/mol
|
0.5 G_PER_L |
CHEBI:16283
L-cystine
|
|
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
2 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Yeast extract | 5 G_PER_L | ||
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
2.5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
variable VARIABLE |
CHEBI:29377
sodium carbonate
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 153 |
| 2026-02-03T11:18:55.717125 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 153 |
| 2026-03-13T03:08:41.514732Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: Na2CO3 |
| 2026-03-13T06:19:25.652513Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004176 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.590715+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FLUID THIOGLYCOLATE medium" to "fluid_thioglycolate_medium" for consistent matching |
| 2026-05-16T09:03:32.060912+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:00.472588+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.898276+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |