Overview
- External ontology
- komodo.medium:161 (source link unavailable) — METHANOMICROBIUM MOBILE medium
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: KOMODO ModelSEED | ID: 161 | DSMZ Medium: 161 (mediadive.medium:161) | Aerobic: Yes Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preserve this provider's exact displayed Gram Amounts and nulls. The 300.00 g/L clarified-rumen-fluid row is a provider assertion, not a conversion from original ml. No density, stereochemistry, missing amount or growth association is inferred. Retain this provider's pH6.5. Its aerobic flag is source metadata, not a verified cultivation condition; the linked original describes anoxic preparation. The linked original uses anoxic preparation and H2/CO2 incubation. This conflicts with KOMODO's aerobic flag; preserve the source distinction rather than certify either as biological growth evidence. Complete inspected source rows define this reconstruction. Archive false LB expansions, flattened quantities and placeholders in append-only history. Preserve earlier supported evidence exactly; unrelated chemical annotations are not recertified.
Composition
flowchart LR
CultureMech_004186["methanomicrobium_mobile_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_004186 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_D_Ca_pantothenate["D-Ca-pantothenate"]
CultureMech_004186 --> ing1_D_Ca_pantothenate
CHEBI_31345["CHEBI:31345"]
ing1_D_Ca_pantothenate -.-> CHEBI_31345
ing2_Lipoic_acid["Lipoic acid"]
CultureMech_004186 --> ing2_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing2_Lipoic_acid -.-> CHEBI_16494
ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004186 --> ing3_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_x_9_H2O -.-> CHEBI_76209
ing4_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
CultureMech_004186 --> ing4_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_Isovaleric_acid["Isovaleric acid"]
CultureMech_004186 --> ing5_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing5_Isovaleric_acid -.-> CHEBI_28484
ing6_CO2["CO2"]
CultureMech_004186 --> ing6_CO2
ing7_Nicotinic_acid["Nicotinic acid"]
CultureMech_004186 --> ing7_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing7_Nicotinic_acid -.-> CHEBI_15940
ing8_Riboflavin["Riboflavin"]
CultureMech_004186 --> ing8_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing8_Riboflavin -.-> CHEBI_17015
ing9_K2HPO4["K2HPO4"]
CultureMech_004186 --> ing9_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4 -.-> CHEBI_131527
ing10_KH2PO4["KH2PO4"]
CultureMech_004186 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_004186 --> ing11_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
ing12_Vitamin_B12["Vitamin B12"]
CultureMech_004186 --> ing12_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing12_Vitamin_B12 -.-> CHEBI_176843
ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004186 --> ing13_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
ing14_Folic_acid["Folic acid"]
CultureMech_004186 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_004186 --> ing15_Thiamine_HCl_x_2_H2O
ing16_Pyridoxine_HCl["Pyridoxine HCl"]
CultureMech_004186 --> ing16_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing16_Pyridoxine_HCl -.-> CHEBI_30961
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004186 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_Resazurin["Resazurin"]
CultureMech_004186 --> ing18_Resazurin
CHEBI_8806["CHEBI:8806"]
ing18_Resazurin -.-> CHEBI_8806
ing19_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_004186 --> ing19_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing19_MnSO4_x_H2O -.-> CHEBI_86364
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_004186 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21__NH4_2SO4["(NH4)2SO4"]
CultureMech_004186 --> ing21__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing21__NH4_2SO4 -.-> CHEBI_62946
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_004186 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_004186 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_H2O["H2O"]
CultureMech_004186 --> ing24_H2O
ing25_KOH["KOH"]
CultureMech_004186 --> ing25_KOH
ing26_2_Methylbutyric_acid["2-Methylbutyric acid"]
CultureMech_004186 --> ing26_2_Methylbutyric_acid
ing27_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_004186 --> ing27_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing27_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing28_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004186 --> ing28_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing28_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing29_Distilled_water["Distilled water"]
CultureMech_004186 --> ing29_Distilled_water
CultureMech_004186 --> more["...13 more"]
Ingredients (43)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.00 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 2 |
D-Ca-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5.00E-5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 3 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5.00E-5 G_PER_L |
CHEBI:16494
lipoic acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 4 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.50 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 5 |
Cysteine-HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.50 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 6 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
0.50 G_PER_L |
CHEBI:28484
isovaleric acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 7 | CO2 | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 8 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5.00E-5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 9 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5.00E-5 G_PER_L |
CHEBI:17015
riboflavin
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 10 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.30 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.30 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 12 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1.80E-3 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 13 | Vitamin B12 | 1.00E-6 G_PER_L |
CHEBI:176843
vitamin B12
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 14 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.16 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Superseded flattening history, not a current amount: [Merged 2 duplicates: 0.12987, 3.0] Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2.00E-5 G_PER_L |
CHEBI:27470
folic acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 16 | Thiamine-HCl x 2 H2O | 5.00E-5 G_PER_L | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. Do not transfer an anhydrous hydrochloride grounding to explicit dihydrate; withdraw copied identity annotations without inventing a replacement ID. | |
| 17 |
Pyridoxine HCl
C8H11NO3.HCl 205.641 g/mol
|
1.00E-4 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
1.00E-4 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 19 |
Resazurin
C12H7NO4 229.191 g/mol
|
1.00E-3 G_PER_L |
CHEBI:8806
Resazurin
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 20 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
5.00E-3 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5.00E-5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 22 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.30 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.00E-4 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.01 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 25 | H2O | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 26 | KOH | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 27 | 2-Methylbutyric acid | 0.50 G_PER_L | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 28 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
3.00E-6 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 29 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.80E-3 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 30 | Distilled water | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 31 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2.00E-5 G_PER_L |
CHEBI:15956
biotin
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 32 | H2 | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. | ||
| 33 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
3.00E-3 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Superseded flattening history, not a current amount: [Merged 2 duplicates: 0.001998, 0.1] Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 34 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.50 G_PER_L |
CHEBI:16135
isobutyric acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 35 |
Valeric acid
C5H10O2 102.133 g/mol
|
0.50 G_PER_L |
CHEBI:17418
valeric acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 36 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
9.00E-3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Superseded flattening history, not a current amount: [Merged 2 duplicates: 0.00799201, 0.1] Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 37 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
3.00E-4 G_PER_L |
CHEBI:34887
nickel dichloride
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 38 | clarified rumen fluid | 300.00 G_PER_L | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. | |
| 39 |
H3BO3
H3BO3 61.833 g/mol
|
1.00E-4 G_PER_L |
CHEBI:33118
boric acid
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 40 |
NaCl
Cl.Na 58.443 g/mol
|
0.61 G_PER_L |
CHEBI:26710
sodium chloride
|
Superseded flattening history, not a current amount: [Merged 2 duplicates: 0.599401, 1.0] Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 41 |
KAl(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
2.00E-4 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 42 | Trypticase (BBL) | 1.00 G_PER_L |
MICRO:0000175 (source link unavailable)
Trypticase peptone
|
Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
| 43 | Yeast extract (Difco) | 1.00 G_PER_L | Exact displayed Gram Amounts and nulls; not rounded molar columns or combined SEED rows. Even clarified-rumen-fluid grams are this provider's assertion, not a density conversion from original ml inputs Exact heading: Gram & Mol amounts are per Liter of medium. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 161 |
| 2026-02-03T11:19:00.197963 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 161 |
| 2026-02-06T05:10:21.280700Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:52.230549Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:25.713798Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004186 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.659810+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOMICROBIUM MOBILE medium" to "methanomicrobium_mobile_medium" for consistent matching |
| 2026-06-05T07:40:21.328905+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.276041+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-22T00:03:26.686152+00:00 | repair_rejected_peptone_groundings.py | CORRECTED_REJECTED_PEPTONE_GROUNDING | CultureMech #484. /ingredients/8/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved. |
| 2026-10-09T09:34:31.181526+00:00 | codex | RESTORED_METHANOFOLLIS_SOURCE_FORMULATIONS_WITH_EVIDENCE | Restore distinct medium161 source formulations, stock scopes, input units and unquantified titrants with exact evidence. Preserve prior supported entries and unknown quantities; correct false duplicate direction and stale paths. No growth association added. |