Overview
- External ontology
- komodo.medium:163_10520 (source link unavailable) — MEDIUM 163 MODIFIED FOR DSM 10520
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- pH buffer: sodium bicarbonate | Source: KOMODO ModelSEED | ID: 163_10520 | DSMZ Medium: 163 (mediadive.medium:163) | Aerobic: No
Composition
flowchart LR
CultureMech_004188["medium_163_modified_for_dsm_10520"]
ing0_NaCl["NaCl"]
CultureMech_004188 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_004188 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_004188 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Na2SO4["Na2SO4"]
CultureMech_004188 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004188 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004188 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Na_DL_lactate["Na-DL-lactate"]
CultureMech_004188 --> ing6_Na_DL_lactate
CHEBI_75228["CHEBI:75228"]
ing6_Na_DL_lactate -.-> CHEBI_75228
ing7_Yeast_extract["Yeast extract"]
CultureMech_004188 --> ing7_Yeast_extract
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_004188 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004188 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_Na_thioglycolate["Na-thioglycolate"]
CultureMech_004188 --> ing10_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing10_Na_thioglycolate -.-> CHEBI_86481
ing11_Ascorbic_acid["Ascorbic acid"]
CultureMech_004188 --> ing11_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing11_Ascorbic_acid -.-> CHEBI_22652
ing12_sodium["sodium"]
CultureMech_004188 --> ing12_sodium
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
25.5102 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.510204 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.02041 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.02041 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.102041 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.04082 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
Na-DL-lactate
C3H5O3.Na 112.06 g/mol
|
2.04082 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 8 | Yeast extract | 1.02041 G_PER_L | ||
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
50 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
10 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 12 | Ascorbic acid | 10 G_PER_L |
CHEBI:22652
ascorbic acid
|
|
| 13 | sodium | variable VARIABLE | Extracted from recipe notes (pH buffer) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 163_10520 |
| 2026-02-03T11:19:06.395441 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 163 |
| 2026-03-13T06:19:25.729304Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004188 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: sodium |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.678411+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 163 MODIFIED FOR DSM 10520" to "medium_163_modified_for_dsm_10520" for consistent matching |
| 2026-05-16T09:04:11.553515+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:15.509133+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.720046+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |