methanosarcina_thermophilic_medium_replace_rumen_fluid_clarified_with_sludge_fluid_medium_119 (CultureMech:004190)

Overview

External ontology

komodo.medium:164_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119) (source link unavailable) — METHANOSARCINA (thermophilic) medium_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119)

Applications

Microbial cultivation

Notes

Archived DSMZ Medium 164 defines METHANOSARCINA (thermophilic) MEDIUM as DSMZ Medium 120 supplemented with 5% (v/v) clarified rumen fluid or sludge fluid from DSMZ Medium 119 and with NaHCO3 increased to 2 g/L; this KOMODO replacement record uses sludge fluid from DSMZ Medium 119.

Composition

flowchart LR
    CultureMech_004190["methanosarcina_thermophilic_medium_replace_rumen_fluid_clarified_wi..."]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_004190 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_004190 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_004190 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004190 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004190 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_NaCl["NaCl"]
    CultureMech_004190 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_004190 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_Biotin["Biotin"]
    CultureMech_004190 --> ing7_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing7_Biotin -.-> CHEBI_15956
    ing8_Folic_acid["Folic acid"]
    CultureMech_004190 --> ing8_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing8_Folic_acid -.-> CHEBI_27470
    ing9_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_004190 --> ing9_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing9_Pyridoxine_HCl -.-> CHEBI_30961
    ing10_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_004190 --> ing10_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing10_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing11_Riboflavin["Riboflavin"]
    CultureMech_004190 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_004190 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_D_Ca_pantothenate["D-Ca-pantothenate"]
    CultureMech_004190 --> ing13_D_Ca_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing13_D_Ca_pantothenate -.-> CHEBI_31345
    ing14_Vitamin_B12["Vitamin B12"]
    CultureMech_004190 --> ing14_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing14_Vitamin_B12 -.-> CHEBI_176843
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_004190 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Lipoic_acid["Lipoic acid"]
    CultureMech_004190 --> ing16_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing16_Lipoic_acid -.-> CHEBI_16494
    ing17_HCl["HCl"]
    CultureMech_004190 --> ing17_HCl
    CHEBI_17883["CHEBI:17883"]
    ing17_HCl -.-> CHEBI_17883
    ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_004190 --> ing18_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing19_ZnCl2["ZnCl2"]
    CultureMech_004190 --> ing19_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing19_ZnCl2 -.-> CHEBI_49976
    ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_004190 --> ing20_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing21_H3BO3["H3BO3"]
    CultureMech_004190 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004190 --> ing22_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing22_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing23_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_004190 --> ing23_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing23_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004190 --> ing24_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004190 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_Yeast_extract["Yeast extract"]
    CultureMech_004190 --> ing26_Yeast_extract
    ing27_Casitone["Casitone"]
    CultureMech_004190 --> ing27_Casitone
    ing28_Resazurin["Resazurin"]
    CultureMech_004190 --> ing28_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing28_Resazurin -.-> CHEBI_8806
    ing29_NaHCO3["NaHCO3"]
    CultureMech_004190 --> ing29_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing29_NaHCO3 -.-> CHEBI_32139
    CultureMech_004190 --> more["...5 more"]

Ingredients (35)

#	Ingredient	Concentration	Ontology	Notes
1	K2HPO4 HO4P.2K 174.174 g/mol	0.348 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	DSMZ/KOMODO Medium 164 sludge variant derives 0.348 G_PER_L K2HPO4 from Archived DSMZ Medium 120.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.227 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	DSMZ/KOMODO Medium 164 sludge variant derives 0.227 G_PER_L KH2PO4 from Archived DSMZ Medium 120.
3	NH4Cl Cl.H4N 53.492 g/mol	0.500 G_PER_L	CHEBI:31206 ammonium chloride	DSMZ/KOMODO Medium 164 sludge variant derives 0.500 G_PER_L NH4Cl from Archived DSMZ Medium 120.
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.500 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	DSMZ/KOMODO Medium 164 sludge variant derives 0.500 G_PER_L MgSO4 x 7 H2O from Archived DSMZ Medium 120.
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.250 G_PER_L	CHEBI:86158 calcium chloride dihydrate	DSMZ/KOMODO Medium 164 sludge variant derives 0.250 G_PER_L CaCl2 x 2 H2O from Archived DSMZ Medium 120.
6	NaCl Cl.Na 58.443 g/mol	2.250 G_PER_L	CHEBI:26710 sodium chloride	DSMZ/KOMODO Medium 164 sludge variant derives 2.250 G_PER_L NaCl from Archived DSMZ Medium 120.
7	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.002 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	DSMZ/KOMODO Medium 164 sludge variant derives 0.002 G_PER_L FeSO4 x 7 H2O from Archived DSMZ Medium 120.
8	Biotin C10H16N2O3S 244.316 g/mol	0.00002 G_PER_L	CHEBI:15956 biotin	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00002 g/L Biotin.
9	Folic acid C19H19N7O6 441.404 g/mol	0.00002 G_PER_L	CHEBI:27470 folic acid	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00002 g/L Folic acid.
10	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.0001 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.0001 g/L Pyridoxine-HCl.
11	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.00005 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00005 g/L Thiamine-HCl x 2 H2O.
12	Riboflavin C17H20N4O6 376.369 g/mol	0.00005 G_PER_L	CHEBI:17015 riboflavin	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00005 g/L Riboflavin.
13	Nicotinic acid C6H5NO2 123.111 g/mol	0.00005 G_PER_L	CHEBI:15940 nicotinic acid	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00005 g/L Nicotinic acid.
14	D-Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.00005 G_PER_L	CHEBI:31345 Calcium pantothenate	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00005 g/L D-Ca-pantothenate.
15	Vitamin B12	0.000001 G_PER_L	CHEBI:176843 vitamin B12	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.000001 g/L Vitamin B12.
16	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.00005 G_PER_L	CHEBI:30753 4-aminobenzoic acid	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00005 g/L p-Aminobenzoic acid.
17	Lipoic acid C8H14O2S2 206.332 g/mol	0.00005 G_PER_L	CHEBI:16494 lipoic acid	DSMZ/KOMODO Medium 164 sludge variant adds 10 mL/L of the Archived DSMZ Medium 141 vitamin stock, yielding 0.00005 g/L Lipoic acid.
18	HCl HCl 36.461 g/mol	0.0025 G_PER_L	CHEBI:17883 hydrogen chloride	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.0025 g/L HCl.
19	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.0015 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.0015 g/L FeCl2 x 4 H2O.
20	ZnCl2 2Cl.Zn 136.296 g/mol	0.00007 G_PER_L	CHEBI:49976 zinc dichloride	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.00007 g/L ZnCl2.
21	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0001 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.0001 g/L MnCl2 x 4 H2O.
22	H3BO3 H3BO3 61.833 g/mol	0.000006 G_PER_L	CHEBI:33118 boric acid	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.000006 g/L H3BO3.
23	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.00019 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.00019 g/L CoCl2 x 6 H2O.
24	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.000002 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.000002 g/L CuCl2 x 2 H2O.
25	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.000024 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.000024 g/L NiCl2 x 6 H2O.
26	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000036 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	DSMZ/KOMODO Medium 164 sludge variant adds 1 mL/L of the Archived DSMZ Medium 320 SL-10 stock, yielding 0.000036 g/L Na2MoO4 x 2 H2O.
27	Yeast extract	2.000 G_PER_L	FOODON:03315426 yeast extract	DSMZ/KOMODO Medium 164 sludge variant derives 2.000 G_PER_L Yeast extract from Archived DSMZ Medium 120.
28	Casitone	2.000 G_PER_L		DSMZ/KOMODO Medium 164 sludge variant derives 2.000 G_PER_L Casitone from Archived DSMZ Medium 120.
29	Resazurin C12H7NO4 229.191 g/mol	0.001 G_PER_L	CHEBI:8806 Resazurin	DSMZ/KOMODO Medium 164 sludge variant derives 0.001 G_PER_L Resazurin from Archived DSMZ Medium 120.
30	NaHCO3 CHO3.Na 84.006 g/mol	2.000 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	DSMZ/KOMODO Medium 164 sludge variant applies 2.000 G_PER_L NaHCO3.
31	Methanol CH4O 32.042 g/mol	10.000 ML_PER_L	CHEBI:17790 methanol	DSMZ/KOMODO Medium 164 sludge variant derives 10.000 ML_PER_L Methanol from Archived DSMZ Medium 120.
32	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.300 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	DSMZ/KOMODO Medium 164 sludge variant derives 0.300 G_PER_L Cysteine-HCl x H2O from Archived DSMZ Medium 120.
33	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.300 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	DSMZ/KOMODO Medium 164 sludge variant derives 0.300 G_PER_L Na2S x 9 H2O from Archived DSMZ Medium 120.
34	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	DSMZ/KOMODO Medium 164 sludge variant derives 1000.000 ML_PER_L Distilled water from Archived DSMZ Medium 120.
35	Sludge fluid (medium 119)	50.000 ML_PER_L		DSMZ/KOMODO Medium 164 sludge variant applies 50.000 ML_PER_L Sludge fluid (medium 119).

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 164_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119)
2026-03-13T06:19:25.738353Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004190
2026-03-17T19:45:40.689096+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOSARCINA (thermophilic) medium_replace_Rumen fluid, clarified_with_Sludge fluid (medium 119)" to "methanosarcina_thermophilic_medium_replace_rumen_fluid_clarified_with_sludge_fluid_medium_119" for consistent matching
2026-08-06T08:58:32.371946+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_164_score35.py	RESOLVED_KOMODO_164_SLUDGE_SCORE35	Archived DSMZ Medium 164 defines METHANOSARCINA (thermophilic) MEDIUM as DSMZ Medium 120 supplemented with 5% (v/v) clarified rumen fluid or sludge fluid from DSMZ Medium 119 and with NaHCO3 increased to 2 g/L; this KOMODO replacement record uses sludge fluid from DSMZ Medium 119.