Overview
- External ontology
- komodo.medium:166 (source link unavailable) — HYPHOMICROBIUM STRAIN X MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: KOMODO ModelSEED | ID: 166 | DSMZ Medium: 166 (mediadive.medium:166) | Aerobic: No
Composition
flowchart LR
CultureMech_004193["hyphomicrobium_strain_x_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_004193 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004193 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NaH2PO4_x_H2O["NaH2PO4 x H2O"]
CultureMech_004193 --> ing2_NaH2PO4_x_H2O
CHEBI_37585["CHEBI:37585"]
ing2_NaH2PO4_x_H2O -.-> CHEBI_37585
ing3_K2HPO4["K2HPO4"]
CultureMech_004193 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Methylamine_hydrochloride["Methylamine hydrochloride"]
CultureMech_004193 --> ing4_Methylamine_hydrochloride
CHEBI_59337["CHEBI:59337"]
ing4_Methylamine_hydrochloride -.-> CHEBI_59337
ing5_Agar["Agar"]
CultureMech_004193 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Na2_EDTA["Na2-EDTA"]
CultureMech_004193 --> ing6_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing6_Na2_EDTA -.-> CHEBI_64734
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004193 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_004193 --> ing8__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing8__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NaH2PO4 x H2O
H2O4P.Na 119.976 g/mol
|
0.5 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.55 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
Methylamine hydrochloride
CH6N.Cl 67.519 g/mol
|
3.4 G_PER_L |
CHEBI:59337
methylamine hydrochloride
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 7 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
50 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
5.54 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
1.1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
Solutions
Trace element solution (Vishniac & Santer, 1957)
Dose: 0.2 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnSO4 x 7 H2O | 22 G_PER_L | |
| MnCl2 x 4 H2O | 5.06 G_PER_L | |
| FeSO4 x 7 H2O | 5 G_PER_L | |
| CuSO4 x 5 H2O | 1.57 G_PER_L | |
| CoCl2 x 6 H2O | 1.61 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 166 |
| 2026-02-03T11:19:05.353073 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 166 |
| 2026-03-13T06:19:25.760777Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004193 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.714019+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYPHOMICROBIUM STRAIN X MEDIUM" to "hyphomicrobium_strain_x_medium" for consistent matching |
| 2026-06-05T07:40:21.370810+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.295584+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T20:44:33.214646+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 1 solution(s): 5 -> 'Trace element solution (Vishniac & Santer, 1957)' @ 0.2 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |