Overview
- External ontology
- komodo.medium:169 (source link unavailable) — SPIROCHAETA ZUELZERAE medium
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- pH buffer: KOH | Source: KOMODO ModelSEED | ID: 169 | DSMZ Medium: 169 (mediadive.medium:169) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004196["spirochaeta_zuelzerae_medium"]
ing0_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004196 --> ing0_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing0_CaCl2_x_2_H2O -.-> CHEBI_86158
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004196 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_004196 --> ing2_Yeast_extract
ing3_D_Glucose["D-Glucose"]
CultureMech_004196 --> ing3_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing3_D_Glucose -.-> CHEBI_17634
ing4_Sodium_resazurin["Sodium resazurin"]
CultureMech_004196 --> ing4_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Sodium_resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_004196 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_Agar["Agar"]
CultureMech_004196 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_NaHCO3["NaHCO3"]
CultureMech_004196 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_K2HPO4["K2HPO4"]
CultureMech_004196 --> ing8_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4 -.-> CHEBI_131527
ing9_KH2PO4["KH2PO4"]
CultureMech_004196 --> ing9_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing9_KH2PO4 -.-> CHEBI_63036
ing10_KOH["KOH"]
CultureMech_004196 --> ing10_KOH
CHEBI_32035["CHEBI:32035"]
ing10_KOH -.-> CHEBI_32035
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0416667 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.520833 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 | Yeast extract | 4.16667 G_PER_L | ||
| 4 |
D-Glucose
C6H12O6 180.156 g/mol
|
2.08333 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 5 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000520833 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.520833 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 | Agar | 10.4167 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for solid medium) |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.39689 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.269448 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 11 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 169 |
| 2026-02-03T11:18:55.898130 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 169 |
| 2026-03-13T03:08:41.895938Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: KOH |
| 2026-03-13T06:19:25.772873Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004196 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.728373+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIROCHAETA ZUELZERAE medium" to "spirochaeta_zuelzerae_medium" for consistent matching |
| 2026-05-16T09:04:32.086676+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:46.395564+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.661795+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |