Overview
- External ontology
- komodo.medium:174 (source link unavailable) — SPIROCHAETA STENOSTREPTA medium
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- pH buffer: KOH | Source: KOMODO ModelSEED | ID: 174 | DSMZ Medium: 174 (mediadive.medium:174) | Aerobic: Yes
Composition
flowchart LR
CultureMech_004203["spirochaeta_stenostrepta_medium"]
ing0_D_Glucose["D-Glucose"]
CultureMech_004203 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Yeast_extract["Yeast extract"]
CultureMech_004203 --> ing1_Yeast_extract
ing2_Peptone["Peptone"]
CultureMech_004203 --> ing2_Peptone
ing3_Sodium_resazurin["Sodium resazurin"]
CultureMech_004203 --> ing3_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Sodium_resazurin -.-> CHEBI_8806
ing4_Na_thioglycolate["Na-thioglycolate"]
CultureMech_004203 --> ing4_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing4_Na_thioglycolate -.-> CHEBI_86481
ing5_Agar["Agar"]
CultureMech_004203 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_KOH["KOH"]
CultureMech_004203 --> ing6_KOH
CHEBI_32035["CHEBI:32035"]
ing6_KOH -.-> CHEBI_32035
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 | Peptone | 2 G_PER_L | ||
| 4 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.5 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 6 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
Optional ingredient (for stabs) |
| 7 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 174 |
| 2026-02-03T11:18:58.058446 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 174 |
| 2026-03-13T03:08:46.930429Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: KOH |
| 2026-03-13T06:19:25.809601Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004203 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.770930+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIROCHAETA STENOSTREPTA medium" to "spirochaeta_stenostrepta_medium" for consistent matching |
| 2026-05-16T09:03:01.540966+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:21.390523+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.301985+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |