Overview
- External ontology
- komodo.medium:182a (source link unavailable) — SULFOLOBUS MT-4 medium
- Applications
-
- Microbial cultivation
- pH
- 3.5
- Notes
- pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 182a | DSMZ Medium: 182a (mediadive.medium:182a) | Aerobic: No
Composition
flowchart LR
CultureMech_004206["sulfolobus_mt_4_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_004206 --> ing0_Yeast_extract
ing1_KH2PO4["KH2PO4"]
CultureMech_004206 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_004206 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004206 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_004206 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_HCl["HCl"]
CultureMech_004206 --> ing5_HCl
CHEBI_17883["CHEBI:17883"]
ing5_HCl -.-> CHEBI_17883
ing6_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_004206 --> ing6_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing6_FeCl2_x_4_H2O -.-> CHEBI_86249
ing7_ZnCl2["ZnCl2"]
CultureMech_004206 --> ing7_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing7_ZnCl2 -.-> CHEBI_49976
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004206 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_004206 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_004206 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004206 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_004206 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004206 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_H2SO4["H2SO4"]
CultureMech_004206 --> ing14_H2SO4
CHEBI_26836["CHEBI:26836"]
ing14_H2SO4 -.-> CHEBI_26836
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 7 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 8 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 182a |
| 2026-02-03T11:19:03.859097 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 182 |
| 2026-03-13T03:09:01.991462Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: H2SO4 |
| 2026-03-13T06:19:25.828148Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004206 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.792850+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFOLOBUS MT-4 medium" to "sulfolobus_mt_4_medium" for consistent matching |
| 2026-05-16T09:04:32.827475+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:47.486137+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.035431+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |