desulfurococcus_medium (CultureMech:004208)

Overview

External ontology

komodo.medium:184 (source link unavailable) — DESULFUROCOCCUS medium

Applications

Microbial cultivation

pH

5.5

Notes

KOMODO Medium 184 expands DSMZ Medium 184 into its metabolite table, including sulfur, salts, trace metals, water, yeast extract, resazurin, nitrogen, and variable H2SO4 for pH 5.5.

Composition

flowchart LR
    CultureMech_004208["desulfurococcus_medium"]
    ing0_Sulfur["Sulfur"]
    CultureMech_004208 --> ing0_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing0_Sulfur -.-> CHEBI_26833
    ing1_KH2PO4["KH2PO4"]
    CultureMech_004208 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
    CultureMech_004208 --> ing2_VOSO4_x_2_H2O
    CHEBI_87009["CHEBI:87009"]
    ing2_VOSO4_x_2_H2O -.-> CHEBI_87009
    ing3_Yeast_extract__Difco_["Yeast extract (Difco)"]
    CultureMech_004208 --> ing3_Yeast_extract__Difco_
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004208 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004208 --> ing5_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_x_9_H2O -.-> CHEBI_76209
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_004208 --> ing6_Yeast_extract
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_004208 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_004208 --> ing8_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing8_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing9__NH4_2SO4["(NH4)2SO4"]
    CultureMech_004208 --> ing9__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing9__NH4_2SO4 -.-> CHEBI_62946
    ing10_N2["N2"]
    CultureMech_004208 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004208 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_004208 --> ing12_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing12_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004208 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_H2O["H2O"]
    CultureMech_004208 --> ing14_H2O
    CHEBI_15377["CHEBI:15377"]
    ing14_H2O -.-> CHEBI_15377
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_004208 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_Resazurin["Resazurin"]
    CultureMech_004208 --> ing16_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing16_Resazurin -.-> CHEBI_8806
    ing17_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_004208 --> ing17_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing17_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing18_CoSO4["CoSO4"]
    CultureMech_004208 --> ing18_CoSO4
    CHEBI_53470["CHEBI:53470"]
    ing18_CoSO4 -.-> CHEBI_53470
    ing19_H2SO4["H2SO4"]
    CultureMech_004208 --> ing19_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing19_H2SO4 -.-> CHEBI_26836

Ingredients (20)

#	Ingredient	Concentration	Ontology	Notes
1	Sulfur S 32.067 g/mol	5.00 G_PER_L	CHEBI:26833 sulfur atom	KOMODO Medium 184 lists 5.00 g/L Sulfur.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.28 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	KOMODO Medium 184 lists 0.28 g/L KH2PO4.
3	VOSO4 x 2 H2O 2H2O.O4S.OV 199.036 g/mol	0.000030 G_PER_L	CHEBI:87009 vanadyl sulfate dihydrate	KOMODO Medium 184 lists 0.000030 g/L VOSO4 x 2 H2O.
4	Yeast extract (Difco)	1.00 G_PER_L		KOMODO Medium 184 lists 1.00 g/L Yeast extract (Difco).
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.07 G_PER_L	CHEBI:86158 calcium chloride dihydrate	KOMODO Medium 184 lists 0.07 g/L CaCl2 x 2 H2O.
6	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.50 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	KOMODO Medium 184 lists 0.50 g/L Na2S x 9 H2O.
7	Yeast extract	1.00 G_PER_L		KOMODO Medium 184 lists 1.00 g/L Yeast extract.
8	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.00180 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	KOMODO Medium 184 lists 0.00180 g/L MnCl2 x 4 H2O.
9	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.000050 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	KOMODO Medium 184 lists 0.000050 g/L CuCl2 x 2 H2O.
10	(NH4)2SO4 2H4N.O4S 132.141 g/mol	1.30 G_PER_L	CHEBI:62946 ammonium sulfate	KOMODO Medium 184 lists 1.30 g/L (NH4)2SO4.
11	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	KOMODO Medium 184 lists nitrogen without gram or molar amounts.
12	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000030 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	KOMODO Medium 184 lists 0.000030 g/L Na2MoO4 x 2 H2O.
13	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	0.02 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	KOMODO Medium 184 lists 0.02 g/L FeCl3 x 6 H2O.
14	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.25 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	KOMODO Medium 184 lists 0.25 g/L MgSO4 x 7 H2O.
15	H2O H2O 18.015 g/mol	variable VARIABLE	CHEBI:15377 water	KOMODO Medium 184 lists water without gram or molar amounts.
16	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.000220 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	KOMODO Medium 184 lists 0.000220 g/L ZnSO4 x 7 H2O.
17	Resazurin C12H7NO4 229.191 g/mol	0.00100 G_PER_L	CHEBI:8806 Resazurin	KOMODO Medium 184 lists 0.00100 g/L Resazurin.
18	Na2B4O7 x 10 H2O 10H2O.H4B4O9.2Na 417.401 g/mol	0.00450 G_PER_L	CHEBI:131366 disodium tetraborate decahydrate	KOMODO Medium 184 lists 0.00450 g/L Na2B4O7 x 10 H2O.
19	CoSO4 Co.O4S 154.996 g/mol	0.000010 G_PER_L	CHEBI:53470 cobalt(II) sulfate	KOMODO Medium 184 lists 0.000010 g/L CoSO4.
20	H2SO4 H2O4S 98.079 g/mol	variable VARIABLE	CHEBI:26836 sulfuric acid	KOMODO Medium 184 lists H2SO4 as the variable pH 5.5 acid.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 184
2026-03-13T06:19:25.834827Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004208
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: H2SO4
2026-03-17T06:30:08.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:40.801510+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "DESULFUROCOCCUS medium" to "desulfurococcus_medium" for consistent matching
2026-05-16T09:03:01.561660+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:21.416197+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00	repair_komodo_184_723_score15.py	RESOLVED_KOMODO_184_723_SCORE15	Replaced the one-row H2SO4 placeholder with the KOMODO Medium 184 metabolite table and kept the DSMZ PDF linked from KOMODO as a reference.