Overview
- External ontology
- komodo.medium:185 (source link unavailable) — THERMOPROTEUS medium
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 185 | DSMZ Medium: 185 (mediadive.medium:185) | Aerobic: No
Composition
flowchart LR
CultureMech_004209["thermoproteus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_004209 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_004209 --> ing1_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing1_FeSO4_x_7_H2O -.-> CHEBI_75836
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_004209 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_004209 --> ing3_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing3_CaSO4_x_2_H2O -.-> CHEBI_32583
ing4_KH2PO4["KH2PO4"]
CultureMech_004209 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_004209 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Yeast_extract["Yeast extract"]
CultureMech_004209 --> ing6_Yeast_extract
ing7_Starch["Starch"]
CultureMech_004209 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
ing8_Sulfur["Sulfur"]
CultureMech_004209 --> ing8_Sulfur
CHEBI_26833["CHEBI:26833"]
ing8_Sulfur -.-> CHEBI_26833
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_004209 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_NaF["NaF"]
CultureMech_004209 --> ing10_NaF
CHEBI_28741["CHEBI:28741"]
ing10_NaF -.-> CHEBI_28741
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_004209 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_004209 --> ing12_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing12_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_004209 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_004209 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_004209 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_004209 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_H2SO4["H2SO4"]
CultureMech_004209 --> ing17_H2SO4
CHEBI_26836["CHEBI:26836"]
ing17_H2SO4 -.-> CHEBI_26836
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.264 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.556 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.492 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.344 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.014 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 | Yeast extract | 0.2 G_PER_L | ||
| 8 | Starch | 5 G_PER_L |
CHEBI:28017
starch
|
|
| 9 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
NaF
F.Na 41.988 g/mol
|
0.84 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.18 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.45 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.005 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.001 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 185 |
| 2026-02-03T11:18:58.638876 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 185 |
| 2026-03-13T03:08:48.408778Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: H2SO4 |
| 2026-03-13T06:19:25.840916Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004209 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.808703+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOPROTEUS medium" to "thermoproteus_medium" for consistent matching |
| 2026-05-16T09:03:01.569737+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:21.425286+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:44.313751+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |