for_dsm_25720

CultureMech:004215 bacterial COMPLEX SOLID_AGAR
Original name: For DSM 25720

Overview

External ontology
komodo.medium:191.2 (source link unavailable) — For DSM 25720
Applications
  • Microbial cultivation
pH
7.5
Notes
KOMODO Medium 191.2 states DSMZ Medium 191 provenance and records pH 7.5 for DSM 25720; DSMZ Medium 191 provides the 50.0 g/L corn meal plus 10.0 g/L agar formulation.

Composition

flowchart LR
    CultureMech_004215["for_dsm_25720"]
    ing0_Corn_meal["Corn meal"]
    CultureMech_004215 --> ing0_Corn_meal
    ing1_Agar["Agar"]
    CultureMech_004215 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Corn meal 50.0 G_PER_L mediadive.compound:2087 (source link unavailable)
Corn meal
 DSMZ Medium 191 blends 50.0 g corn meal in 800 ml distilled water, leaves it overnight in the refrigerator, heats it at 60 C for one hour, filters it, and brings the filtrate to 1 L.
2 Agar 10.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 191 adds 10.0 g agar after bringing the corn-meal filtrate to 1 L and heats to dissolve it before autoclaving.

Preparation

  1. Blend 50.0 g corn meal in 800 ml distilled water and leave overnight in the refrigerator.
  2. Heat at 60 C for one hour.
  3. Filter and bring the volume to 1 L.
  4. Add 10.0 g agar and heat to dissolve.
  5. Autoclave the completed corn meal agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 191.2
2026-02-03T11:19:10.603473 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 191
2026-03-13T03:09:20.151794Z data-quality-cleanup-v1.0 Data quality fixes Added pH buffer: NaOH
2026-03-13T06:19:25.861884Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004215
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-17T06:30:28.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:40.832908+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "For DSM 25720" to "for_dsm_25720" for consistent matching
2026-05-16T09:03:34.329764+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:04.101586+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_komodo_corn_meal_score25.py RESOLVED_KOMODO_CORN_MEAL_SCORE25_GRAPH KOMODO Medium 191.2 states DSMZ Medium 191 provenance and records pH 7.5 for DSM 25720; DSMZ Medium 191 provides the 50.0 g/L corn meal plus 10.0 g/L agar formulation.