Overview
- External ontology
- komodo.medium:191.3 (source link unavailable) — For DSM 25945
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- KOMODO Medium 191.3 states DSMZ Medium 191 provenance and records pH 7.4 for DSM 25945; DSMZ Medium 191 provides the 50.0 g/L corn meal plus 10.0 g/L agar formulation.
Composition
flowchart LR
CultureMech_004216["for_dsm_25945"]
ing0_Corn_meal["Corn meal"]
CultureMech_004216 --> ing0_Corn_meal
ing1_Agar["Agar"]
CultureMech_004216 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Corn meal | 50.0 G_PER_L |
mediadive.compound:2087 (source link unavailable)
Corn meal
|
DSMZ Medium 191 blends 50.0 g corn meal in 800 ml distilled water, leaves it overnight in the refrigerator, heats it at 60 C for one hour, filters it, and brings the filtrate to 1 L. |
| 2 | Agar | 10.0 G_PER_L |
CHEBI:2509
agar
|
DSMZ Medium 191 adds 10.0 g agar after bringing the corn-meal filtrate to 1 L and heats to dissolve it before autoclaving. |
Preparation
- Blend 50.0 g corn meal in 800 ml distilled water and leave overnight in the refrigerator.
- Heat at 60 C for one hour.
- Filter and bring the volume to 1 L.
- Add 10.0 g agar and heat to dissolve.
- Autoclave the completed corn meal agar.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:02.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 191.3 |
| 2026-02-03T11:19:13.742998 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 191 |
| 2026-03-13T06:19:25.864353Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:004216 |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:40.835920+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For DSM 25945" to "for_dsm_25945" for consistent matching |
| 2026-06-05T07:41:04.108893+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-10T00:00:00-07:00 | repair_komodo_corn_meal_score25.py | RESOLVED_KOMODO_CORN_MEAL_SCORE25_GRAPH | KOMODO Medium 191.3 states DSMZ Medium 191 provenance and records pH 7.4 for DSM 25945; DSMZ Medium 191 provides the 50.0 g/L corn meal plus 10.0 g/L agar formulation. |