medium_194_modified_for_dsm_5092 (CultureMech:004233)

Overview

External ontology

komodo.medium:194_5092 (source link unavailable) — MEDIUM 194 MODIFIED FOR DSM 5092

Applications

Microbial cultivation

pH range

{'min': 7.1, 'max': 7.4}

Notes

Source: KOMODO ModelSEED | ID: 194_5092 | DSMZ Medium: 194 (mediadive.medium:194) | Aerobic: No

Composition

flowchart LR
    CultureMech_004233["medium_194_modified_for_dsm_5092"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_004233 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_KH2PO4["KH2PO4"]
    CultureMech_004233 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_004233 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_004233 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_004233 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_004233 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004233 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Resazurin["Resazurin"]
    CultureMech_004233 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_NaHCO3["NaHCO3"]
    CultureMech_004233 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_004233 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_HCl["HCl"]
    CultureMech_004233 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_004233 --> ing11_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing12_ZnCl2["ZnCl2"]
    CultureMech_004233 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_004233 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_004233 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004233 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_004233 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_004233 --> ing17_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004233 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Folic_acid["Folic acid"]
    CultureMech_004233 --> ing19_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing19_Folic_acid -.-> CHEBI_27470
    ing20_Biotin["Biotin"]
    CultureMech_004233 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Pyridoxine_HCl["Pyridoxine HCl"]
    CultureMech_004233 --> ing21_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_HCl -.-> CHEBI_30961
    ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_004233 --> ing22_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing23_Riboflavin["Riboflavin"]
    CultureMech_004233 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_004233 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_D_Ca_pantothenate["D-Ca-pantothenate"]
    CultureMech_004233 --> ing25_D_Ca_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_D_Ca_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_004233 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_004233 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_004233 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494
    ing29_putrescine["putrescine"]
    CultureMech_004233 --> ing29_putrescine
    CHEBI_17148["CHEBI:17148"]
    ing29_putrescine -.-> CHEBI_17148

Ingredients (30)

#	Ingredient	Concentration	Ontology	Notes
1	Na2SO4 2Na.O4S 142.043 g/mol	3.00 G_PER_L	CHEBI:32149 sodium sulfate	KOMODO Medium 194_5092 lists 3.00 g/L Na2SO4 in the final-component table.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.20 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	KOMODO Medium 194_5092 lists 0.20 g/L KH2PO4 in the final-component table.
3	NH4Cl Cl.H4N 53.492 g/mol	0.30 G_PER_L	CHEBI:31206 ammonium chloride	KOMODO Medium 194_5092 lists 0.30 g/L NH4Cl in the final-component table.
4	NaCl Cl.Na 58.443 g/mol	1.00 G_PER_L	CHEBI:26710 sodium chloride	KOMODO Medium 194_5092 lists 1.00 g/L NaCl in the final-component table.
5	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.40 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	KOMODO Medium 194_5092 lists 0.40 g/L MgCl2 x 6 H2O in the final-component table.
6	KCl Cl.K 74.551 g/mol	0.50 G_PER_L	CHEBI:32588 potassium chloride	KOMODO Medium 194_5092 lists 0.50 g/L KCl in the final-component table.
7	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.15 G_PER_L	CHEBI:86158 calcium chloride dihydrate	KOMODO Medium 194_5092 lists 0.15 g/L CaCl2 x 2 H2O in the final-component table.
8	Resazurin C12H7NO4 229.191 g/mol	0.000999 G_PER_L	CHEBI:8806 Resazurin	KOMODO Medium 194_5092 lists 0.000999 g/L Resazurin in the final-component table.
9	NaHCO3 CHO3.Na 84.006 g/mol	5.00 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	KOMODO Medium 194_5092 lists 5.00 g/L NaHCO3 in the final-component table.
10	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.40 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	KOMODO Medium 194_5092 lists 0.40 g/L Na2S x 9 H2O in the final-component table.
11	HCl HCl 36.461 g/mol	0.000671 G_PER_L	CHEBI:17883 hydrogen chloride	KOMODO Medium 194_5092 lists 0.000671 g/L HCl in the final-component table.
12	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.00149 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	KOMODO Medium 194_5092 lists 0.00149 g/L FeCl2 x 4 H2O in the final-component table.
13	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000698 G_PER_L	CHEBI:49976 zinc dichloride	KOMODO Medium 194_5092 lists 0.0000698 g/L ZnCl2 in the final-component table.
14	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000997 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	KOMODO Medium 194_5092 lists 0.0000997 g/L MnCl2 x 4 H2O in the final-component table.
15	H3BO3 H3BO3 61.833 g/mol	0.00000598 G_PER_L	CHEBI:33118 boric acid	KOMODO Medium 194_5092 lists 0.00000598 g/L H3BO3 in the final-component table.
16	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000189 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	KOMODO Medium 194_5092 lists 0.000189 g/L CoCl2 x 6 H2O in the final-component table.
17	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.00000199 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	KOMODO Medium 194_5092 lists 0.00000199 g/L CuCl2 x 2 H2O in the final-component table.
18	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000239 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	KOMODO Medium 194_5092 lists 0.0000239 g/L NiCl2 x 6 H2O in the final-component table.
19	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000359 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	KOMODO Medium 194_5092 lists 0.0000359 g/L Na2MoO4 x 2 H2O in the final-component table.
20	Folic acid C19H19N7O6 441.404 g/mol	0.0000200 G_PER_L	CHEBI:27470 folic acid	KOMODO Medium 194_5092 lists 0.0000200 g/L Folic acid in the final-component table.
21	Biotin C10H16N2O3S 244.316 g/mol	0.0000200 G_PER_L	CHEBI:15956 biotin	KOMODO Medium 194_5092 lists 0.0000200 g/L Biotin in the final-component table.
22	Pyridoxine HCl C8H11NO3.HCl 205.641 g/mol	0.0000999 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	KOMODO Medium 194_5092 lists 0.0000999 g/L Pyridoxine HCl in the final-component table.
23	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.0000500 G_PER_L	CHEBI:132751 thiamine hydrochloride dihydrate	KOMODO Medium 194_5092 lists 0.0000500 g/L Thiamine-HCl x 2 H2O in the final-component table.
24	Riboflavin C17H20N4O6 376.369 g/mol	0.0000500 G_PER_L	CHEBI:17015 riboflavin	KOMODO Medium 194_5092 lists 0.0000500 g/L Riboflavin in the final-component table.
25	Nicotinic acid C6H5NO2 123.111 g/mol	0.0000500 G_PER_L	CHEBI:15940 nicotinic acid	KOMODO Medium 194_5092 lists 0.0000500 g/L Nicotinic acid in the final-component table.
26	D-Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.0000500 G_PER_L	CHEBI:31345 Calcium pantothenate	KOMODO Medium 194_5092 lists 0.0000500 g/L D-Ca-pantothenate in the final-component table.
27	Vitamin B12	0.000000999 G_PER_L	CHEBI:176843 vitamin B12	KOMODO Medium 194_5092 lists 0.000000999 g/L Vitamin B12 in the final-component table.
28	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.0000500 G_PER_L	CHEBI:30753 4-aminobenzoic acid	KOMODO Medium 194_5092 lists 0.0000500 g/L p-Aminobenzoic acid in the final-component table.
29	Lipoic acid C8H14O2S2 206.332 g/mol	0.0000500 G_PER_L	CHEBI:16494 lipoic acid	KOMODO Medium 194_5092 lists 0.0000500 g/L Lipoic acid in the final-component table.
30	putrescine C4H12N2 88.154 g/mol	0.90 G_PER_L	CHEBI:17148 putrescine	DSMZ Medium 194 specifies 0.90 g/L putrescine for DSM 5092 as a replacement for sodium propionate.

Preparation

DSMZ Medium 194 sparges Solution A with an 80% N2 and 20% CO2 gas mixture to reach pH below 6, distributes it under the same gas atmosphere into anoxic Hungate-type tubes or serum vials, and autoclaves it.
DSMZ Medium 194 autoclaves Solution B under 80% N2 and 20% CO2, autoclaves Solutions C and E under 100% N2, filter-sterilizes Solution D under 100% N2, then adds Solutions B to E to sterile cooled Solution A in sequence.
DSMZ Medium 194 adjusts the final pH to 7.1-7.4.
DSMZ Medium 194 notes that growth of some strains may be stimulated with 10-20 mg/L sodium dithionite from a freshly prepared anoxic 5% w/v solution sterilized by filtration.
For DSM 5092, DSMZ Medium 194 replaces sodium propionate with 0.90 g/L putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:02.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 194_5092
2026-02-03T11:19:09.887080	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 194
2026-03-13T06:19:26.067318Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:004233
2026-03-17T06:30:26.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:41.069028+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MEDIUM 194 MODIFIED FOR DSM 5092" to "medium_194_modified_for_dsm_5092" for consistent matching
2026-06-05T07:41:15.582611+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:01.743862+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00	repair_dsmz_194_desulfobulbus_score15.py	RESOLVED_DSMZ_KOMODO_194_SCORE15	Replaced stale DSMZ Medium 194a Desulfovirga rows with the DSMZ Medium 194 DSM 5092 putrescine substitution.