trace_element_solution_medium_882

CultureMech:004325 bacterial DEFINED LIQUID
Original name: Trace element solution (medium 882)

Overview

External ontology
komodo.medium:2052 (source link unavailable) — Trace element solution (medium 882)
Applications
  • Microbial cultivation
pH
1.8
Notes
KOMODO 2052 lists Trace element solution (medium 882) as a defined submedium at pH 1.8. Its metabolite table contains 0.07 g/L ZnCl2, 0.07 g/L CuCl2 x 2 H2O, 0.06 g/L CoCl2 x 6 H2O, 0.06 g/L MnCl2 x 2 H2O, 0.03 g/L H3BO3, 0.01 g/L Na2MoO4, variable H2SO4, and H2O.

Composition

flowchart LR
    CultureMech_004325["trace_element_solution_medium_882"]
    ing0_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004325 --> ing0_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing0_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing1_ZnCl2["ZnCl2"]
    CultureMech_004325 --> ing1_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing1_ZnCl2 -.-> CHEBI_49976
    ing2_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
    CultureMech_004325 --> ing2_MnCl2_x_2_H2O
    CHEBI_131395["CHEBI:131395"]
    ing2_MnCl2_x_2_H2O -.-> CHEBI_131395
    ing3_H2O["H2O"]
    CultureMech_004325 --> ing3_H2O
    CHEBI_15377["CHEBI:15377"]
    ing3_H2O -.-> CHEBI_15377
    ing4_Na2MoO4["Na2MoO4"]
    CultureMech_004325 --> ing4_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing4_Na2MoO4 -.-> CHEBI_75215
    ing5_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_004325 --> ing5_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing5_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing6_H2SO4["H2SO4"]
    CultureMech_004325 --> ing6_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing6_H2SO4 -.-> CHEBI_26836
    ing7_H3BO3["H3BO3"]
    CultureMech_004325 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.06 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
 KOMODO Medium 2052 lists 0.06 g/L CoCl2 x 6 H2O.
2 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
 KOMODO Medium 2052 lists 0.07 g/L ZnCl2.
3 MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
0.06 G_PER_L CHEBI:131395
manganese(II) chloride dihydrate
 KOMODO Medium 2052 lists 0.06 g/L MnCl2 x 2 H2O.
4 H2O
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 KOMODO Medium 2052 lists H2O without a gram amount; normalized here as the solvent for 1 L of Trace element solution (medium 882).
5 Na2MoO4
MoO4.2Na 205.916 g/mol
0.01 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
 KOMODO Medium 2052 lists 0.01 g/L Na2MoO4.
6 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.07 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 KOMODO Medium 2052 lists 0.07 g/L CuCl2 x 2 H2O.
7 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 KOMODO Medium 2052 lists H2SO4 without a gram amount and also identifies H2SO4 as the pH adjuster for pH 1.8.
8 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
 KOMODO Medium 2052 lists 0.03 g/L H3BO3.

Preparation

  1. Prepare the 1 L Trace element solution stock from H2O, H2SO4, and the cobalt, zinc, manganese, molybdate, copper, and borate salts listed by KOMODO 2052.
  2. Adjust the Trace element solution stock to pH 1.8 with H2SO4 as needed.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 2052
2026-03-13T06:19:26.588370Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004325
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:41.699422+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Trace element solution (medium 882)" to "trace_element_solution_medium_882" for consistent matching
2026-05-16T09:04:37.579983+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:53.948226+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_komodo_2052_score15.py RESOLVED_KOMODO_2052_SCORE15 Replaced the H2SO4-only pH-buffer import with the complete KOMODO 2052 metabolite table; preserved H2SO4 as a variable listed component and pH adjuster; and grounded all disclosed components through MediaIngredientMech.