wolfes_mineral_elixir_medium_792

CultureMech:004386 bacterial DEFINED LIQUID
Original name: Wolfes Mineral Elixir (medium 792)

Overview

External ontology
komodo.medium:2106 (source link unavailable) — Wolfes Mineral Elixir (medium 792)
Applications
  • Microbial cultivation
pH
1.0
Notes
KOMODO Medium 2106 is a SubMedium import for the Wolfe's mineral elixir printed inside DSMZ Medium 792. DSMZ Medium 792 / MediaDive solution 1605 lists fourteen salt rows, 1000.00 ml distilled water, and a pH 1.0 adjustment with diluted H2SO4 for this stock.

Composition

flowchart LR
    CultureMech_004386["wolfes_mineral_elixir_medium_792"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_004386 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_004386 --> ing1_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing1_MnSO4_x_H2O -.-> CHEBI_86364
    ing2_NaCl["NaCl"]
    CultureMech_004386 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_004386 --> ing3_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing4_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_004386 --> ing4_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing4_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_004386 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_004386 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_004386 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_004386 --> ing8_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing8_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing9_H3BO3["H3BO3"]
    CultureMech_004386 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_004386 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_004386 --> ing11__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing11__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing12_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_004386 --> ing12_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing13_Na2SeO4["Na2SeO4"]
    CultureMech_004386 --> ing13_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing13_Na2SeO4 -.-> CHEBI_77775
    ing14_Distilled_water["Distilled water"]
    CultureMech_004386 --> ing14_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing14_Distilled_water -.-> CHEBI_15377
    ing15_H2SO4["H2SO4"]
    CultureMech_004386 --> ing15_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing15_H2SO4 -.-> CHEBI_26836

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
30.00 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 30.00 g MgSO4 x 7 H2O.
2 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
5.00 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 5.00 g MnSO4 x H2O.
3 NaCl
Cl.Na 58.443 g/mol
10.00 G_PER_L CHEBI:26710
sodium chloride
 DSMZ Medium 792 Wolfe's mineral elixir lists 10.00 g NaCl.
4 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.00 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 1.00 g FeSO4 x 7 H2O.
5 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
1.80 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 1.80 g CoCl2 x 6 H2O.
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.00 G_PER_L CHEBI:86158
calcium chloride dihydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 1.00 g CaCl2 x 2 H2O.
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.80 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 1.80 g ZnSO4 x 7 H2O.
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.10 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 0.10 g CuSO4 x 5 H2O.
9 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 0.18 g AlK(SO4)2 x 12 H2O.
10 H3BO3
H3BO3 61.833 g/mol
0.10 G_PER_L CHEBI:33118
boric acid
 DSMZ Medium 792 Wolfe's mineral elixir lists 0.10 g H3BO3.
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.10 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 0.10 g Na2MoO4 x 2 H2O.
12 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.80 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 2.80 g (NH4)2Ni(SO4)2 x 6 H2O.
13 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.10 G_PER_L CHEBI:63939
sodium tungstate dihydrate
 DSMZ Medium 792 Wolfe's mineral elixir lists 0.10 g Na2WO4 x 2 H2O.
14 Na2SeO4
2Na.O4Se 188.936 g/mol
0.10 G_PER_L CHEBI:77775
sodium selenate
 DSMZ Medium 792 Wolfe's mineral elixir lists 0.10 g Na2SeO4.
15 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 792 Wolfe's mineral elixir lists 1000.00 ml distilled water.
16 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 DSMZ Medium 792 instructs first adjusting Wolfe's mineral elixir to pH 1.0 with diluted H2SO4; the amount is retained as variable because it is titrated to pH.

Preparation

  1. First adjust the Wolfe's mineral elixir stock to pH 1.0 with diluted H2SO4.
  2. Add and dissolve the Wolfe's mineral elixir salts in 1000.0 ml distilled water.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:02.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 2106
2026-03-13T06:19:26.713668Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:004386
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:41.852938+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Wolfes Mineral Elixir (medium 792)" to "wolfes_mineral_elixir_medium_792" for consistent matching
2026-05-16T09:04:38.881353+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:55.492358+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-13T00:00:00-07:00 repair_komodo_2106_wolfes_mineral_elixir_score15.py RESOLVED_KOMODO_2106_WOLFES_MINERAL_ELIXIR_SCORE15 KOMODO Medium 2106 is a SubMedium import for the Wolfe's mineral elixir printed inside DSMZ Medium 792. DSMZ Medium 792 / MediaDive solution 1605 lists fourteen salt rows, 1000.00 ml distilled water, and a pH 1.0 adjustment with diluted H2SO4 for this stock. Replaced the imported H2SO4-only pH-buffer stub with the complete DSMZ Wolfe's mineral elixir formula, corrected water to 1000.0 ml/L, and grounded the pH-adjustment row.